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Study on the pathogenesis of osteoarthrosis secondary to scraping the surface of the articular cartilage : with special interest on the importance of the surface layr of the articular cartilage

刮擦关节软骨表面继发骨关节病发病机制的研究：特别关注关节软骨表层的重要性

基本信息

批准号：
08671649
负责人：
KOBAYASHI Seneki
金额：
$ 1.6万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671649/
关键词：
articular cartilage osteoarthrosis knee joint surface layr rabbit lubrication proteoglycan

项目摘要

The surface layr of the articular cartilage, which is devoid of collagen fibrils and cells, contributes to the lubrication of the joint and protects the cartilage from digestion by exogenous enzymes. The layr is mainly composed of proteoglycan, a major constituent of the cartilage. When the surface layyr was removed from the articular cartilage by scraping the articular surface of the rabbit knee, osteoarthrosis frequently developed in the joint. The development of osteoarthrosis was dependent on the scraped area. When the scraped area was large, the incidence of the development of osteoarthrosis was high. The purpose of the present study was to elucidate the pathogenesis of osteoarthrosis, especially the relationship between the development of osteoarthrosis and the surface layr of the articular cartilage. Two groups of rabbits were examined. In one group, only central one-third of the femoral articular surface was scraped in the right knee. In the other, almost the entire articular s … More urface of the femoral condyles was scraped. In the former group, animals did not develop osteoarthrosis in their right knees, while rabbits in the latter group frequently developed osteoarthrosis. In the cryo-scanning electron microscopy, in the group of the small scraped area, the surface layr was regenerated in the scraped area with in a week. At that point of time, lubrication of the joint, which had been impaired by the scraping, was restored to the normal level. Immuno-chemical examination revealed that the regenerated surface layr in the scraped area was composed of proteoglycan as the layr in the normal cartilage. Transmission electron microscopy showed enrichment of intracellular organs, indicating enhanced production of extracellular matrix by the chondrocytes in and around the scraped area. In the group in which almost entire articular surface of the knee joint was scraped, animals frequently developed osteoarthrosis of the knee joint. In the scraped area, the surface layr was not observed. The impaired lubrication of the joint was not improved, and the degenerative changes progressed. These results indicated that the development of osteoarthrosis after scraping the articular surface was related to the restoration of the surface layr which was dependent on the scraped area. When the scraped area was small, the surface layr is regenerated by activated secretion of chondrocytes and progressive osteoarthrotic changes do not occur. When the scraped area was large, the surface layr was not restored by the cells and the articular cartilage undergo progressive degeneration and destruction. Less

关节软骨的表层缺乏胶原纤维和细胞，有助于关节的润滑并保护软骨免受外源性酶的消化。层主要由蛋白聚糖组成，蛋白聚糖是软骨的主要成分。当通过刮擦兔膝关节的关节面从关节软骨去除表面层时，关节中经常发生骨关节病。骨关节病的发生与刮伤面积有关。当刮伤面积较大时，骨关节病的发生率较高。本研究旨在阐明骨关节病的发病机制，特别是骨关节病的发生发展与关节软骨表层的关系。对两组家兔进行了检查。在一组中，仅刮擦右膝股骨关节面的中心三分之一。在另一个，几乎整个关节 ...更多信息刮除股骨髁表面。在前一组中，动物的右膝没有发生骨关节病，而后一组中的兔子经常发生骨关节病。冷冻扫描电镜观察，小面积刮伤组，刮伤区一周内表面层再生。此时，因刮擦而受损的关节润滑恢复到正常水平。免疫组化结果显示，刮伤区再生软骨表面层与正常软骨表面层一样由蛋白多糖组成。透射电子显微镜显示细胞内器官的富集，表明刮擦区域内和周围的软骨细胞产生的细胞外基质增强。在膝关节的几乎整个关节面被刮擦的组中，动物经常发生膝关节骨关节病。在刮擦区域中，未观察到表面层。关节润滑受损未得到改善，退行性变化进展。结果提示，刮除关节面后骨关节病的发生与关节面的修复有关，而关节面的修复又依赖于刮除面积。当刮伤面积较小时，表面层通过软骨细胞的活化分泌而再生，并且不发生进行性骨关节病改变。当刮伤面积较大时，表层细胞不能修复，关节软骨发生进行性退变和破坏。少