ROLES OF ADHESION MOLECULE CD44 ON RHEUMATOID CHONDROCYTES IN ARTHRITIS

粘附分子 CD44 对关节炎中类风湿软骨细胞的作用

• 批准号: 08671673
• 负责人: TAKAGI Toshitaka
• 金额: $ 1.47万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671673/
• 关键词: CD44; adhesion molecule; rheumatoid arthritis; chondrocyte; immunohistochemistly; collagen induced arthritis; flowcytometly; air pouch; 変形性関節症; コラーゲン関節炎; 軟骨変性

1. EXPRESSION OF CD44 ON RHEUMATOID CHONDROCYTESImmunohistochemical analysis revealed that average of CD44-positive chondrocytes in rheumatoid arthritis (RA) was 93%, which was significantly (p<0.05) higher than that of osteoarthritis (OA)(59%), and that of femoral head in femoral neck fracture (46%). When the isoform of CD44 was studied, haemopoietic form was dominant in RA and OA.Flowcytometry revealed that average of CD44-positive chondrocytes is RA, OA and normal was 39%, 52% and 10%, respectively, after 24 hours in suspension culture.2. EXPRESSION OF CD44 ON CHONDROCYTE OF KNEE IN MICE WITH COLLAGEN INDUCED ARTHRITISExpression of CD44 on chondrocytes was observed in the area of metachromasy loss in toluidin blue staining in knee of mice after 3 weeks of immunization with type II collagen.3. EFFECTS OF HYALURONATE ON INFLAMMATION IN RAT AIR-POUCHHyaluronate, a ligand of CD44, inhibited the synthesis of IL-1 and TNF- α from lining of rat air-pouch with inflammation induced by lipopolysaccharide.

1.CD44在类风湿软骨细胞中的表达免疫组织化学结果显示，CD44阳性细胞在类风湿关节炎(RA)中的平均表达为93%，显著高于骨性关节炎(OA)的59%和股骨颈骨折的股骨头(46%)(P<0.05)。结果：1.研究CD44亚型时，RA和OA以造血细胞为主，悬浮培养24小时后，CD44阳性的软骨细胞平均为RA、OA和正常软骨细胞的39%、52%和10%。胶原性关节炎小鼠膝关节软骨细胞CD44的表达II型胶原免疫3周后，在小鼠膝关节甲苯胺蓝染色异染区观察到CD44的表达。透明质酸对大鼠气囊炎症的影响透明质酸能抑制内毒素致炎大鼠气囊衬里IL-1和肿瘤坏死因子-α的合成。

项目成果

N.KUROSAWA、T.TAKAGI: "EFFECT OF HYALURONATE ON CD44 EXPRESSION OF INFILTRATING CELL IN EXUDATE OF RAT AIR-POUCH"J RHEUMATOL. IN PRESS.

N.KUROSAWA、T.TAKAGI：“透明质酸对大鼠气囊渗出液中浸润细胞 CD44 表达的影响”J RHEUMATOL 出版社。

M.I..SATO,T.TAKAGI: "EXPRESSION OF CD44 IN THE AREA OF PROTEOGLYCAN LOSS SIROT 99"FREUND PUBLISHING HOUSE LTD. 768 (589-594) (1999)

M.I..SATO,T.TAKAGI：“CD44 在蛋白聚糖损失 SIROT 99 区域的表达”FREUND Publishing HOUSE LTD。

N. KUROSAKA, T. TAKAGI: "EFFECTS OF HYALURONATE ON CD44 EXPRESSION OF INFILTRATING CELL IN EXUDATE OF RAT AIR-POUCH INDUCED BY SENSITIZATION WITH LIPOPOLYSACCHARIDE"J RHEUMATOL. (IN PRESS).

N. Kurosaka, T. TAKAGI：“透明质酸对脂多糖致敏诱导的大鼠气囊渗出液中浸润细胞 CD44 表达的影响”J RHEUMATOL。

M. I. SATO, T. TAKAGI: "EXPRESSION OF CD44 IN THE AREA OF PROTEOGLYCAN LOSS IN THE EARLY STAGE OF TYPE II COLLAGEN-INDUCED ARTHRITIS IN MICE SIROT 99 ED BY H. STEIN"FREUND PUBLISHING HOUSE ISBNPP. 589-594 (1999)

M. I. SATO，T. TAKAGI：“小鼠 SIROT 99 版小鼠 II 型胶原诱导关节炎早期蛋白聚糖丢失区域中 CD44 的表达”，H. Stein 编着”FREUND 出版社 ISBNPP。

MI.Sato,T.Koshino,T.Takagi: "CD44 expression on chondrocytes in Knees of DBA/1 mice with tyoe II collagen-induced arthritis" Clinical and Eyperimental Rheumatology. in press.

MI.Sato、T.Koshino、T.Takagi：“患有 tyoe II 胶原诱导关节炎的 DBA/1 小鼠膝关节软骨细胞上的 CD44 表达”临床和实验风湿病学。