PERIPHERAL NERVE REGENERATION IN MAGNETIC STIMULATION

磁刺激中的周围神经再生

• 批准号: 08671693
• 负责人: DEZAWA Akira
• 金额: $ 0.64万
• 依托单位: TEIKYO UNIVERSITY SCHOOL OF MEDICINE DEPARTMENT OF ORTHOPAEDIC SURGERY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671693/
• 关键词: nerve regeneration; gap junction; connexin; biocytin; dye coupling; magnetic field; シュワン細胞間; blocytin; サイトカイン; gap iunction; biocytin; 坐骨神経; 磁場; HRP; 後根神経節; Schwan cell; 神経再生; ZO-1

Gap junctions are intercellular channels which mediate the traffic of ions and a variety of molecular messengers between contiguous cells. Here, we report on the possibility that atypical gap junctions develop between heterologous tissues, that is the regenerating nerve axon and Schwann cell, during peripheral nerve regeneration of adult rats. After a complete transection and subsequent regeneration in the rat sciatic nerve distal segment, a small scale of gap junction-like structure was observed between the regenerating axon and adjoining Schwann cell. One of the gap junctional protein, connexln32, located at a small region of contact site between the axon and Schwann cell in an immunoelectrori microscopyy. The intercellular coupling was examined by evidence that blocytin, a dye of small molecular weight, was transported from regenerating axons into adjoining Schwann cells. The present findings suggest that regenerating axons communicate directly with adjacent Schwann cells through small gap junctions, which may play a role in the mechanism of regeneration following nerve transection,

间隙连接是细胞间通道，介导相邻细胞之间的离子和各种分子信使的运输。在这里，我们报告了在成年大鼠周围神经再生过程中异源组织（即再生神经轴突和雪旺细胞）之间形成非典型间隙连接的可能性。在大鼠坐骨神经远端段完全横断并随后再生后，在再生轴突和相邻雪旺细胞之间观察到小规模的间隙连接样结构。在免疫电镜下，间隙连接蛋白之一 connexln32 位于轴突和雪旺细胞之间的接触位点的小区域。细胞间偶联通过以下证据进行检查：布洛西丁（一种小分子量染料）从再生轴突转运到相邻的雪旺细胞中。目前的研究结果表明，再生轴突通过小间隙连接直接与邻近的雪旺细胞通讯，这可能在神经横断后的再生机制中发挥作用，

项目成果

M.A.Dezawa: "Tight junctions between the axon & Schwancell during PNS regenerates" Neuro Report. 1. 1829-1832 (1996)

M.A.Dezawa：“轴突之间的紧密连接

M.Dezawa, T.Mutoh, A.Dezawa: "Putative gap junctional communication between axon and regencrating Schwann cells during mammalianperipheral nerve regeneration" Neuroscience. 85. 663-667 (1998)

M.Dezawa、T.Mutoh、A.Dezawa：“哺乳动物周围神经再生过程中轴突和再生雪旺细胞之间假定的间隙连接通讯”神经科学。