Experimental study of extracorporeal lung assist in rat

大鼠体外肺辅助实验研究

• 批准号: 08671751
• 负责人: OKAMOTO Taisuke
• 金额: $ 1.47万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671751/
• 关键词: Rat; Extracorporeal Circulation; Gas Exchange

We investigated the effects of V-V and V-A bypass extracorporeal lung assist (ECLA) using a miniature membrane oxygenators (Mini-ECLA system) in rats. Wistar rats underwent general anesthesia with intraperitoneal pentobarbital administration (50 mg/kg) and tracheostomy for ventilation. V-V and V-A ECLA were carried out through the right atrium via a jugular vein using a 6 Fr.Atom catheter or 18 G Instite catheter for drainage, respectively, and return flow through the femoral vein or common carotid artery, respectively, using a 24G Therflow catheter. We used a miniature dense-membrane, hollow-fiber oxygenator which was developed for rat (priming volume 4 ml). Total priming volume in mini-ECLA circuits was 20 ml.We maintained the V-A ECLA for a maximum of 150 min (mean duration : 83(]SY.+-。[)61 min) and obtained bypass flow of 35.0(]SY.+-。[)3.3 ml/kg/min (mean(]SY.+-。[)SD). Analysis of blood gases between inlet and outlet of the oxygenator revealed that high oxygenation and CO<@D22@>D2 removal were obtained in the oxygenator ; gas exchange was well maintained during ECLA.The values of hemoglobin, plasma protein concentration and hematocrit at 5 min after ECLA start were 6.8 g/dl, 4.0 g/dl and 18.3 %, respectively, and were stable during the ECLA.The values were significantly decreased compared to the pre-ECLA values due to hemodilution by the relatively large priming volume for rat.Furthermore, hyperkalemia that occurred in V-A ECLA was likely due to hemolysis by the roller pump, however other electrolytes were not significantly changed. In future work we need to construct smaller circuits to prevent hemodilution and improve drainage to avoid hyperkalemia due to hemolysis during ECLA.This mini-ECLA system achieved to prolonged time on ECLA in rats and may be useful for investigation of the effects of extracorporeal circulation.

本研究应用微型膜式氧合器（Mini-ECLA系统）对大鼠进行V-V和V-A旁路体外肺辅助（ECLA）。Wistar大鼠腹腔注射戊巴比妥（50 mg/kg）进行全身麻醉，气管切开通气。V-V和V-A ECLA分别使用6 Fr.Atom导管或18 G Instite导管经颈静脉通过右心房进行引流，并使用24 G Therflow导管分别通过股静脉或颈总动脉回流。我们使用了一个小型的致密膜，中空纤维氧合器，这是为大鼠（预充量4毫升）。微型ECLA回路中的总预充量为20 ml。我们维持V-A ECLA最长150 min（平均持续时间：83（]SY ±）。[)61 min），获得了35.0（μ S·±·min）的旁路流量。[)3.3 ml/kg/min（平均值（±）.（SD）。氧合器入口和出口之间的血气分析表明，氧合器中获得了高氧合和CO<@D22@>D2去除; ECLA开始后5 min血红蛋白、血浆蛋白浓度和红细胞压积分别为6.8g/dl、4.0g/dl和18.3%，与ECLA前相比，由于大鼠相对较大的预充量导致血液稀释，这些值显著降低。此外，V-A ECLA中发生的高钾血症可能是由于滚子泵溶血所致，但其他电解质没有显著变化。在今后的工作中，我们需要构建更小的回路，以防止血液稀释和改善引流，以避免在ECLA过程中由于溶血引起的高钾血症。这种微型ECLA系统实现了延长大鼠的ECLA时间，可能有助于体外循环效果的研究。