The study of cytokines, as a regulator on the proliferation, invasion and differentiation of trophoblasts.

研究细胞因子作为滋养细胞增殖、侵袭和分化的调节因子。

• 批准号: 08671921
• 负责人: SEKI Hiroyuki
• 金额: $ 1.41万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671921/
• 关键词: Trophoblast; decidua; immortalized cell line; PGE2; progesterone; DNA; cytokines; TNF-alpha; IL-1β; VEGF; TGF-β; b-FGF; PyNPase活性; 脱落膜細胞; 絨毛細胞; サイトカイン; プロゲステロン; 増殖; 分化; 進入; 不死化細胞

The vector ZipSV4O-6, a recombinant retrovirus derived from ZipNeoSV(X) carrying the SV40 large T gene and the marker gene neomycin acetyl transferase were used to infect primary trophoblast and decidua derived cells, respectivelly. (1)TNF-alpha dose-dependently decreased DNAsynthesis without any effect on progesterone synthe-sis. (2)IL-beta has no effect on both DNA and progesterone synthesis. (3)TCL conditioned medium decreased PGE2 synthesis by DE-1 without affecting DNA content in DE-1. (4)DE-1 conditioned medium increased progesterone synthesis by TCL, whereas that decreased DNA content in TCL.(5)THP-1, which is the cell line derived from monocytic leukemia, attenuated the action of DE-1 on progesterone synthesis and DNA content in TCL.These results indicate that TNF-a regulates the function and proliferation both of decidual and trophoblast cells, that trophoblasts inhibit PGE2 synthesis by decidual cells withou affecting proliferation of decidual cells, and that decidual cells inhibit proliferation of trophoblasts whereas that stimulate progesterone synthesis by trophoblasts. Moreover, it was indicated that THP-1 had effects on the mutual actions between trophoblasts and decidual cells.

用含有SV 40大T基因和标记基因新霉素乙酰转移酶的ZipNeoSV（X）重组逆转录病毒载体ZipSV 4 O-6分别感染原代滋养层细胞和蜕膜细胞。（1）TNF-α剂量依赖性地降低DNA合成，对孕酮合成无影响。（2）IL-β对DNA和孕酮的合成均无影响。（3）TCL条件培养液对DE-1细胞DNA含量无明显影响，但可抑制DE-1细胞PGE_2的合成。（4）DE-1条件培养液可促进TCL细胞孕酮的合成，但可降低TCL细胞DNA含量。（5）单核细胞白血病细胞系THP-1可减弱DE-1对TCL细胞孕酮合成和DNA含量的影响，提示TNF-α调节蜕膜细胞和滋养层细胞的功能和增殖，滋养层细胞抑制蜕膜细胞合成PGE_2，但不影响蜕膜细胞的增殖，蜕膜细胞抑制滋养层细胞的增殖，而刺激滋养层细胞合成孕酮。提示THP-1对滋养细胞和蜕膜细胞之间的相互作用有影响。

项目成果

Lewis, M.P. et al.: "Partial characterisation of an immortalized trophoblast cell-line, TCL-1, which possesses a CSF-1 auto crine loop." Placenta. 17. 137-146 (1996)

刘易斯，M.P.

Lewis, M.P., Clements, M., Takeda, S., Kirby, P.L., Seki, H., Lonsdale, L.B., Sallivan, M.H.F., Elder, M.G.& White, J.O.: "Partial characterisation of an immortalized trophoblast cell-line, TCL-1, which possesses a CSF-1 autocrine loop." Placenta. 17. 137

Lewis, M.P.、Clements, M.、Takeda, S.、Kirby, P.L.、Seki, H.、Lonsdale, L.B.、Sallivan, M.H.F.、Elder, M.G.

関 博之他: "絨毛細胞と脱落膜細胞の相互作用" 産婦人科の世界. 49. 621-627 (1997)

Hiroyuki Seki 等：“绒毛膜细胞和蜕膜细胞之间的相互作用”《妇产科世界》49. 621-627 (1997)。

Seki, H.et al.: "The regulation of progesterone and hCG production from placental cells by interleukin-1B." Biochim.Biophys.Acta. 1336. 342-348 (1997)

Seki, H. 等人：“白细胞介素 1B 对胎盘细胞黄体酮和 hCG 产生的调节。”

Seki, H., Zosmer, A., Elder, M.G.and Sullivan, M.H.F.: "The regulation of progesterone and hCG production from placental cells by interleukin-1beta." Biochim.Biophys.Acta. 1336. 342-348 (1997)

Seki, H.、Zosmer, A.、Elder, M.G. 和 Sullivan, M.H.F.：“白细胞介素 1β 对胎盘细胞黄体酮和 hCG 产生的调节。”