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Direct Effects of Anesthertics and Vasoconstrictors on Contraction in Aorta Smooth Muscle of Spontaneously Hypertensive Rats

麻醉药和血管收缩药对自发性高血压大鼠主动脉平滑肌收缩的直接影响

基本信息

批准号：
08672164
负责人：
SATO Masahito
金额：
$ 1.41万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The aim of this study is to clarify the direct effects of a variety of anesthetics and vasoconstrictors on contraction and intracellular CaィイD12+ィエD1 mobilization in aorta smooth muscle of spontaneously hypertensive rats. Particularly, dental local anesthetics contains high concentration with epinephrine. Furthermore, we know dental patients with essential hypertension treated with nonselective β adorenoceptor blocking agents may show remarkable increase in blood pressure after administration of dental local anesthetics containing 1/80000 epinephrine. The hemodynamic changes should occur as a net result due to interaction between epinephrine with β adorenoceptor blocking agents. We investigated the effects of epinephrine alone or the combination of epinephrine and nonselective β adorenoceptor blocking agents, propranolol, on isometric tension of spontaneously hypertensive rat(SHR) aorta smooth muscle and intracellular calcium level ( [CaィイD12+ィエD1] i ) by the fura-2/AM simultaneously, and compared with those of normotensive Wister Kyoto rat(WKY).The results were obtained as follows ;1. In WKY, the increases in tension and [ CaィイD12+ィエD1 ] i induced by 10ィイD1-6ィエD1 M epinephrine were less than those by 60 mM KCl.2. In WKY, the tension induced by the combination of epinephrine and propranolol was significantly increased more than that by epinephrine alone, but there were no differences between them of [ CaィイD12+ィエD1 ] i.3. In SHR, the increase in tension induced by epinephrine was larger than that in WKY, but there were no differences between them of [ CaィイD12+ィエD1 ] i. By the combination of epinephrine and propranolol, [ CaィイD12+ィエD1 ] i was increased more than that by epinephrine alone, but the tension was maintained at the same level as that induced by epinephrine alone.

本研究的目的是阐明各种麻醉剂和血管收缩剂对自发性高血压大鼠主动脉平滑肌收缩和细胞内CaィイD12+ィD1动员的直接影响。特别是牙科局部麻醉剂中含有高浓度的肾上腺素。此外，我们知道患有原发性高血压的牙科患者在使用非选择性β肾上腺素受体阻滞剂治疗后，在使用含有1/80000肾上腺素的牙科局部麻醉剂后可能会出现血压显着升高。由于肾上腺素与β肾上腺素受体阻断剂之间的相互作用，血流动力学变化应该作为最终结果发生。我们通过fura-2/AM研究单独使用肾上腺素或联合使用肾上腺素和非选择性β肾上腺素能受体阻断剂普萘洛尔对自发性高血压大鼠(SHR)主动脉平滑肌等长张力和细胞内钙水平([CaィイD12+ィD1] i)的影响，并与正常血压Wister进行比较。京都大鼠(WKY)。所得结果如下： 1．在WKY中，10ィイD1-6ィエD1 M肾上腺素引起的张力和[CaィイD12+ィエD1] i 的增加小于60 mM KCl.2引起的增加。在WKY中，肾上腺素和普萘洛尔组合引起的张力比单独使用肾上腺素显着增加，但[CaィイD12+ィエD1] i.3之间没有差异。在SHR中，肾上腺素引起的张力增加比WKY中更大，但[CaィイD12+ィエD1]i之间没有差异。通过肾上腺素和普萘洛尔的组合，[CaィイD12+ィエD1]i比单独使用肾上腺素增加更多，但张力维持在与单独使用肾上腺素引起的相同水平。