Simplified measurements of the allergic released metallic ions within the oral cavit

本研究的目的是评价一种新的金属离子释放测定方法，以阐明变态反应的机制。与传统的金属修复体EPMA分析方法相比，该方法具有操作简单、节省时间等优点。首先，确定了测定口腔内金属离子释放的条件。基础实验考察了分光光度计法、电光度计法和目视比色法3种测量装置。因此，选择了分光光度测量方法。其次，对6名上颌牙列佩戴金属咬合夹板的受试者进行了临床实验，实验夹板材料为镍铬合金和钴铬合金，被认为是变态反应率较高的牙科材料。结果表明：1)镍铬合金中铬离子的平均释放量为0.139 mg/l(最大0.293 mg/l，最小0.06 mg/l)。2)镍铬合金的平均析出量为0.654 mg/l(最大为1.094 mg/l，最小为0.496 mg/l)。3)钴铬合金中铬离子的平均释放量为0.08 mg/l(最大为0.141 mg/l，最小为0.022 mg/l)。

The purpose of this study was to evaluate a new measuring method of released metallic ions, in order to clarify the mechanism of allergy. As compared with the conventional method by EPMA analysis of metal restorations, the advantages of this method are simplicity of handling and time saving. Firstly, it was determined the conditions nesessary for measurements of the released metallicions within the oral cavity. The basic experiment examined 3 types of measuring device, such as spectrophotometer, electrophotometer and visual colorimetric way. As a result, spectrophotometric measurement was selected. Secondly, the clinical experiments involed 6 subjects wearing occlusal mental splint on the maxillary dentition and the experimental splints were prepared Ni-Cr alloy and Co-Cr alloy which were considered to be dental materials having a high allergy rate. After lateral jaw movement during 5 minutes, it was attempted to judge on released metallic ions with the spectrophotometer and reagent set for water analyzer.THe results were as follows : 1) The average volumes of Cr-ion released from Ni-Cr alloy were 0.139mg/1 (maximum : 0.293mg/1, minimum : 0.06mg/1). 2) The average volumes of Ni-ion released from Ni-Cr alloy were 0.654mg/1 (maximum : 1.094mg/1, minimum : 0.496mg/1). 3) The average volumes of Cr-ion released from Co-Cr alloy were 0.08mg/1 (maximum : 0.141mg/1, minimum : 0.022mg/1).In conclusion, it can be said that the simplified measurements of released metallic ions were capable for clinical judgment on condition that were applied full mouth restrations.