Simplified measurements of the allergic released metallic ions within the oral cavit

口腔内过敏性释放金属离子的简化测量

• 批准号: 08672248
• 负责人: FURUKAWA Kazutoshi
• 金额: $ 1.28万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672248/
• 关键词: metal allergy; released metallic ion; simplified measurement; spectrophotometer; water analyzer; nickel ion; chromium ion; 口腔内イオン濃度; 簡易測定法; 機能的測定条件

The purpose of this study was to evaluate a new measuring method of released metallic ions, in order to clarify the mechanism of allergy. As compared with the conventional method by EPMA analysis of metal restorations, the advantages of this method are simplicity of handling and time saving. Firstly, it was determined the conditions nesessary for measurements of the released metallicions within the oral cavity. The basic experiment examined 3 types of measuring device, such as spectrophotometer, electrophotometer and visual colorimetric way. As a result, spectrophotometric measurement was selected. Secondly, the clinical experiments involed 6 subjects wearing occlusal mental splint on the maxillary dentition and the experimental splints were prepared Ni-Cr alloy and Co-Cr alloy which were considered to be dental materials having a high allergy rate. After lateral jaw movement during 5 minutes, it was attempted to judge on released metallic ions with the spectrophotometer and reagent set for water analyzer.THe results were as follows : 1) The average volumes of Cr-ion released from Ni-Cr alloy were 0.139mg/1 (maximum : 0.293mg/1, minimum : 0.06mg/1). 2) The average volumes of Ni-ion released from Ni-Cr alloy were 0.654mg/1 (maximum : 1.094mg/1, minimum : 0.496mg/1). 3) The average volumes of Cr-ion released from Co-Cr alloy were 0.08mg/1 (maximum : 0.141mg/1, minimum : 0.022mg/1).In conclusion, it can be said that the simplified measurements of released metallic ions were capable for clinical judgment on condition that were applied full mouth restrations.

本研究旨在探讨一种新的金属离子释放测量方法，以阐明过敏的机制。与传统的金属修复体EPMA分析方法相比，该方法操作简单，节省时间。首先，确定了测量口腔内金属释放量所需的条件。基础实验考察了分光光度计、电光度计和目视比色法三种测量装置。因此，选择分光光度法测定。其次，6名受试者在上颌列佩戴咬合精神夹板进行临床实验，实验夹板分别制备了Ni-Cr合金和Co-Cr合金，这两种材料被认为是过敏率较高的牙科材料。在侧颌运动5分钟后，尝试用分光光度计和水分析仪试剂对释放的金属离子进行判断。结果表明：1)Ni-Cr合金中Cr-ion的平均释放体积为0.139mg/1（最大0.293mg/1，最小0.06mg/1）；2) Ni-Cr合金中ni离子的平均释放体积为0.654mg/1，最大值为1.094mg/1，最小值为0.496mg/1。3) Co-Cr合金中Cr-ion的平均释放体积为0.08mg/1，最大值为0.141mg/1，最小值为0.022mg/1。综上所述，简化后的金属离子释放量可用于全口限制条件下的临床判断。