Determination of salivary antiviral antibodies (HSV, HCV, HIV) in Oral

本文尝试测定唾液中对单纯疱疹病毒（HSV）、丙型肝炎病毒（HCV）和人类免疫缺陷病毒（HIV）等致病性病毒的特异性抗体。现将目前所取得的资料加以说明。【唾液HIV抗体的测定】对46例疑似HIV感染者的唾液和血清进行免疫印迹技术和ELISA检测。用0.5 ml灭菌的1%生理盐水刺激后，从每位受试者收集两毫升混合唾液。当血清或唾液中出现GP160、GP110/120和GP41两条或两条以上条带时，判断HIV抗体阳性。46份血清样品中，31份经Western blot检测为阳性，15份为阴性。与这些结果相比，ELISA检测唾液样品的结果的符合率为100%。由于血清和唾液检测结果的一致性，进一步证明了唾液中HIV抗体的测定是可行的。【唾液HSV抗体的测定】采用血清中和抗体测定技术（NT）、ELISA法和改良ELISA法（ABC-ELISA），尝试测定唾液HSV特异性抗体。收集了129名志愿者和193名医学生、护学生的唾液和血清样本。在129份志愿者血清样本中，3种方法均为阳性45份，阴性84份。采用ELISA和ABC-ELISA检测唾液样品，ELISA结果与血清样品的符合率为87.6%。为提高灵敏度，将ELISA法与亲和素-生物素过氧化物酶法相结合，建立了ABC-ELISA法。结果，同意率提高到95.3%。193例医、护生血清样本的符合率为95.9%。【唾液HCV抗体的测定】对35份HCV阳性血清样本和3份HCV阴性血清样本分别采用ELISA和ABC-ELISA法测定唾液HCV特异性抗体。ELISA法和ABC-ELISA法检测结果的符合率分别为50.0%和94.7%。少

The determination of antibodies specific to pathogenic viruses including herpes simplex virus (HSV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) in saliva was attempted. The data obtained so far are presented.[Determination of salivary HIV antibody] HIV antibody was determined by the Western blot technique and ELISA in both salivary and serum samples obtained from 46 subjects suspected to be infected by HIV. Two milliliters of mixed saliva was collected from each subject after stimulation with 0.5 ml of sterilized l% saline. HIV antibody was judged to be positive when two or more bands of GP160, GP110/120, and GP41 appeared on the serum or salivary Western blot pattern. Of the 46 serum samples, 31 were judged to be positive, and 15 to be negative, by the Western blot technique. Compared with these results, the percent agreement of the results of examination of the salivary samples by the ELISA was 100%. Because of this agreement of the results in serum and saliva, t … More he determination of the salivary HIV antibody was concluded to be possible.[Determination of salivary HSV antibody] The determination of salivary HSV-specific antibody was attempted by the technique for the determination of serum neutralizing antibodies (NT), ELISA, and modified ELISA using an avidin-biotin system (ABC-ELISA). Salivary and serum samples were obtained from 129 volunteers and 193 medical students and nursing students. Of the 129 serum samples from volunteers, 45 were positive, and 84 were negative, by all 3 methods. The salivary samples were examined by the ELISA and ABC-ELISA, and the percent agreement of the results of the ELISA was 87.6% compared with the results in the serum samples. To increase the sensitivity, an ABC-ELISA method was developed by combining the ELISA with the avidin-biotin peroxidase method. As a result, the percent agreement improved to 95.3%. In the 193 samples from medical students and nursing students, the percent agreement with the results in the serum samples was 95.9%.[Determination of salivary HCV antibody] Salivary HCV-specific antibody was determined by the ELISA and ABC-ELISA in 35 samples from the subjects with HCV-positive serum samples and 3 samples from those with HCV-negative serum samples. The percent agreement of the results between the serum and salivary samples was 50.0% by the ELISA and 94.7% by the ABC-ELISA. Less