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Determination of salivary antiviral antibodies (HSV, HCV, HIV) in Oral

口腔中唾液抗病毒抗体（HSV、HCV、HIV）的测定

基本信息

批准号：
08672321
负责人：
HAYASHI Seiichi
金额：
$ 1.73万
依托单位：
Yokohama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672321/
关键词：
HSV HCV HIV ELISA techniques ABC-ELISA technique Western blot technique Saliva Salivary antiviral antibodies ELISA ABC-ELISA ウイルス抗体口腔感染症唾液中の抗体測定 HSV抗体 HCV抗体唾液中の抗体

项目摘要

The determination of antibodies specific to pathogenic viruses including herpes simplex virus (HSV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) in saliva was attempted. The data obtained so far are presented.[Determination of salivary HIV antibody] HIV antibody was determined by the Western blot technique and ELISA in both salivary and serum samples obtained from 46 subjects suspected to be infected by HIV. Two milliliters of mixed saliva was collected from each subject after stimulation with 0.5 ml of sterilized l% saline. HIV antibody was judged to be positive when two or more bands of GP160, GP110/120, and GP41 appeared on the serum or salivary Western blot pattern. Of the 46 serum samples, 31 were judged to be positive, and 15 to be negative, by the Western blot technique. Compared with these results, the percent agreement of the results of examination of the salivary samples by the ELISA was 100%. Because of this agreement of the results in serum and saliva, t … More he determination of the salivary HIV antibody was concluded to be possible.[Determination of salivary HSV antibody] The determination of salivary HSV-specific antibody was attempted by the technique for the determination of serum neutralizing antibodies (NT), ELISA, and modified ELISA using an avidin-biotin system (ABC-ELISA). Salivary and serum samples were obtained from 129 volunteers and 193 medical students and nursing students. Of the 129 serum samples from volunteers, 45 were positive, and 84 were negative, by all 3 methods. The salivary samples were examined by the ELISA and ABC-ELISA, and the percent agreement of the results of the ELISA was 87.6% compared with the results in the serum samples. To increase the sensitivity, an ABC-ELISA method was developed by combining the ELISA with the avidin-biotin peroxidase method. As a result, the percent agreement improved to 95.3%. In the 193 samples from medical students and nursing students, the percent agreement with the results in the serum samples was 95.9%.[Determination of salivary HCV antibody] Salivary HCV-specific antibody was determined by the ELISA and ABC-ELISA in 35 samples from the subjects with HCV-positive serum samples and 3 samples from those with HCV-negative serum samples. The percent agreement of the results between the serum and salivary samples was 50.0% by the ELISA and 94.7% by the ABC-ELISA. Less

尝试测定唾液中的致病病毒特异性抗体，包括单纯疱疹病毒（HSV）、丙型肝炎病毒（HCV）和人类免疫缺陷病毒（HIV）。现将目前获得的数据列出。 [唾液HIV抗体的测定] 采用Western blot技术和ELISA对46名疑似HIV感染者的唾液和血清样本中的HIV抗体进行测定。在用0.5ml无菌1％盐水刺激后，从每个受试者收集2毫升混合唾液。当血清或唾液Western blot图谱上出现两条或两条以上GP160、GP110/120、GP41条带时，判定HIV抗体阳性。通过Western blot技术，46份血清样本中，31份被判定为阳性，15份被判定为阴性。与这些结果相比，通过ELISA对唾液样品进行检查的结果的一致性百分比为100%。由于血清和唾液中的结果一致，因此可以确定唾液中的 HIV 抗体。[唾液 HSV 抗体的测定] 通过血清中和抗体 (NT) 测定技术、ELISA 和使用抗生物素蛋白-生物素系统的改良 ELISA 技术，尝试测定唾液 HSV 特异性抗体 (ABC-ELISA)。唾液和血清样本取自 129 名志愿者和 193 名医学生和护理生。通过所有 3 种方法，在来自志愿者的 129 份血清样本中，45 份呈阳性，84 份呈阴性。对唾液样本进行ELISA和ABC-ELISA检测，ELISA结果与血清样本结果的一致性为87.6%。为了提高灵敏度，将 ELISA 与亲和素-生物素过氧化物酶法相结合，开发了 ABC-ELISA 方法。结果，一致性百分比提高到 95.3%。 193份医学生和护生样本中，与血清样本结果一致率为95.9%。【唾液HCV抗体测定】采用ELISA和ABC-ELISA法，对35份HCV阳性血清样本和3份HCV阴性血清样本中的唾液HCV特异性抗体进行检测。 ELISA 法血清与唾液样本结果的一致性为 50.0%，ABC-ELISA 法结果的一致性为 94.7%。较少的