Investigation of Usefulness of Histoculture Drug Response Assay in Oral Cancer

组织培养药物反应测定在口腔癌中的有效性研究

• 批准号: 08672343
• 负责人: YANAI Chie
• 金额: $ 0.9万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672343/
• 关键词: Oral cancer; Histoculture drug response assay; Cheomosensitivity; oral squamous cell carcinoma; MTT-assay; Oral cancer

Histoculture drug response assay was performed in the 19 patients with advanced oral squamous cell carcinoma (23 specimens) at the department of oral surgery, and its usefulness was assessed. In this assay, drugs dorrved including cisplatin, carboplatin and adriamycin were used. Specimens from a primary carcinoma biopsy, and carcinoma tissue specimens during operation surgical were cultured in contact with the drugs on collagen gel for 7 days. More than 50% carcinoma-inhibiting effects by the drugs in MTT assay to the control was considered useful. Drugs were cut off at the concentration of 20 mug/ml of CDDP, 25 mug/ml of CBDCA, and 15 mug/ml of ADM.This histoculture drug response assay could keep inter cellular contact during culture. The effect of the drugs with MTT assay was assessable in 91.3% of cases of oral squamous cell carcinoma, as confirmed with MTT assay. Primary region, metastasis lymph node and each parts had no different inhibition index. Althrough there was no significant differnce between chemotherapy and non-chemotherapy based on CDDP or CBDCA in 10 assesable cases, this could be one of the could be one of anticancer drug choises. This method appears to be useful as a clinical test.

对口腔外科19例（23例）晚期口腔鳞状细胞癌患者进行组织培养药物反应试验，评价其临床应用价值。在该试验中，使用了包括顺铂、卡铂和阿霉素在内的药物。将来自原发性癌活检的标本和手术期间的癌组织标本与胶原凝胶上的药物接触培养7天。MTT法测定的抑瘤率超过对照组的50%，则认为是有效的。以20 μ g/ml的CDDP、25 μ g/ml的CBDCA和15 μ g/ml的ADM为药物阻断浓度。用MTT法检测药物对口腔鳞癌的疗效，其阳性率为91.3%。原发区、转移淋巴结及各部位的抑制指数无差异。在10例可评价的病例中，以CDDP或CBDCA为基础的化疗与非化疗之间无显著性差异，这可能是抗癌药物的选择之一。这种方法作为临床试验似乎是有用的。