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Study of the Culture of Human Odontoclasts in Serum Obtained from Low Calcium-Diet Rabbits and Root Resorption of Deciduous Teeth

低钙兔血清中人破牙细胞培养与乳牙牙根吸收的研究

基本信息

批准号：
08672404
负责人：
OGASAWARA Yasushi
金额：
$ 1.09万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672404/
关键词：
Low calcium-diet Serum Odontoclast Cell culture Root resorption 乳歯歯根吸収

项目摘要

Odontoclasts are morphologically quite similar to osteoclasts but details on the difference of both cells is still not clear. There have also been no report on the cultivation of human odontclasts. The purpose of this study was to cultivate the cells obtained from the resorbed root surfaces of the human deciduous teeth with the serum obtained from low calcium-diet rabbits, One hundred and fifty-two human deciduous teeth in physiologic root resorption were used in this study. The cells were cultivated on bone slices with serum obtained from normal-diet rabbits, serum obtained from low calcium-diet rabbits, or serum obtained from low calcium-diet rabbits containing 10^<-8>mol salmon calcitonin. After the cultivation, tartrate-resistant acid phosphatase(TRAP) activity was detected using a light microscope. The resorption lacunae on bone slices were examined with a scanning electron microscope. The results were as follows;1. A decrease of the TRAP positive cells were observed, when normal-diet rabbit serum was used.2. A great increase of the multinuclear TRAP positive cells was observed on the seventh day, when the serum obtained from the low calcium-diet rabbits was used.3. The appearance of TRAP active cells was completely inhibited by calcitonin.4. The cultured cells with serum obtained from low calcium-diet rabbits were capable of bone resorption.In conclusion, it is considered that the cultured multinuclear TRAP positive cells were mature odontoclasts indused from the odontoclast progenitors of human deciduous teeth and this culture system was useful for the culture of human odontoclast progenitors.

破牙细胞在形态上与破骨细胞非常相似，但两种细胞的差异细节仍不清楚。目前也没有关于人类破牙细胞培养的报道。本研究的目的是用低钙饮食兔的血清培养人乳牙生理性根吸收的根面细胞，共152颗。用正常饲料兔血清、低钙饲料兔血清或含10摩尔鲑鱼降钙素的低钙饲料兔血清在骨片上培养细胞。培养结束后，在光学显微镜下检测抗酒石酸酸性磷酸酶(TRAP)活性。用扫描电子显微镜观察骨片上的吸收陷窝。实验结果如下：1.加入正常饲料兔血清后，TRAP阳性细胞数明显减少。用低钙饲料兔血清培养第7天，多核TRAP阳性细胞明显增多。降钙素可完全抑制TRAP活性细胞的出现。结论：培养的多核TRAP阳性细胞为成熟的破牙细胞，其来源为人乳牙破牙细胞前体细胞，该培养体系可用于人破牙细胞前体细胞的培养。