Stimulation of phagocytosis and phagosome-lysosome fusion by glycolipids from Grifola frondasa (Maitake).

灰树花 (舞茸) 的糖脂刺激吞噬作用和吞噬体-溶酶体融合。

• 批准号: 08680067
• 负责人: MIYAZAKI Yoshiko
• 金额: $ 0.83万
• 依托单位: Mukogawa Women's University Junior College Division
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680067/
• 关键词: Grifola frondosa (Maitake); glycolipids; phagocytosis; phagosome-lysosome fusion; superoxide anion; glycosphingolipid

In this report, I attempted to elucidate the relationship between the structure of glycolipids from Grifola frondosa (Maitake) and the mechnism of phagocytic processes in human monocytes (MN) or polymorphonuclear leukocytes (PMN). The characteristic lipid components of Grifola frondosa (Maitake) were cardiolipin (CL), phosphatidylserine (PS) phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic Acid (PA), phosphatidylcholine (PC), phosphatidyl-ethanolamine (PE), and three classes of glycolipids (MGL-1,2,3). The structures of glycolipids from Maitake were determined by thin layr chromatography (TLC), Infrared spectrometry, FAB mass spectrometry and NMR spectrometry. The effect of glycolipids on phagocytosis by human PMN or MN using killed Staphylococcus aureus cells coated with glycolipids (MGL-1,2,3) was examined. The rate of phagosome-lysosome fusion by PMN was determined by counting acridine orange-stained bacteria under a fluorescence microscope. Superoxide anion production was determined by the reduction of cytochrome c. Both phagocytosis and phagosome-lysosome fusion of MGL-2 was stimulated in dose-dependent manner, while MGL-1 and MGL-3 did not. Relative phagosome-lysosome fusion rate of MGL-2-coated staphylococci was six times higher than that of the noncoated control and phagocytic rate was twice, respectively. The superoxide anion release from PMN was not affected by MGL-2. I compared the effect of lipopolysaccharide (LPS) from Salmonella enteritidis with that of MGL-2 from Maitake. The glycolipid (MGL-2) reversed the inhibition of phagocytosis by LPS.

在这篇报道中，我试图阐明灰树花（Grifola frondosa，灰树花）糖脂结构与人单核细胞（MN）或多形核白细胞（PMN）吞噬过程机制的关系。灰树花的主要脂质成分为心磷脂（CL）、磷脂酰丝氨酸（PS）、磷脂酰胆碱（PC）、磷脂酰肌醇（PI）、磷脂酸（PA）、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和3类糖脂（mgl -1,2,3）。采用薄层色谱法、红外光谱法、FAB质谱法和核磁共振谱法对舞茸糖脂的结构进行了分析。用糖脂包被金黄色葡萄球菌（mgl -1,2,3）的死亡细胞研究了糖脂对人PMN或MN吞噬作用的影响。通过荧光显微镜下吖啶橙染色的细菌计数来测定吞噬体-溶酶体与PMN融合的速率。超氧阴离子的产生是由细胞色素c的减少决定的。MGL-2的吞噬和吞噬体-溶酶体融合都以剂量依赖性的方式受到刺激，而MGL-1和MGL-3则没有。mgl -2包被葡萄球菌的吞噬体与溶酶体的相对融合率是未包被对照的6倍，吞噬率是未包被对照的2倍。MGL-2对PMN的超氧阴离子释放没有影响。我比较了肠炎沙门氏菌脂多糖（LPS）和舞茸MGL-2的作用。糖脂（MGL-2）逆转了LPS对吞噬的抑制作用。

项目成果

Miyazaki, Y., Oka, S., Mizuno, S.and Yano, I.: "Stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from Sphingomonas paucimobilis." J.Biochem. 118. 271-277 (1995)

Miyazaki, Y.、Oka, S.、Mizuno, S. 和 Yano, I.：“少动鞘氨醇鞘糖脂对吞噬作用和吞噬体-溶酶体融合的刺激。”

Miyazaki, Y., Oka, S., Hara-Hotta, H.and Yano, I.: "Stimulation and inhibition of polymorphonuclear leukocytes phagocytosis by lipoamino acids isolated from S.marcescens." FEMS Immunology and medical Microbiology.6. 265-272 (1993)

Miyazaki, Y.、Oka, S.、Hara-Hotta, H. 和 Yano, I.：“从粘质沙门氏菌中分离出的脂氨基酸对多形核白细胞吞噬作用的刺激和抑制”。

Kawai, G.: "Molecular species of cerebrosides in fruiting bodies of Lentinus edodes and their biological activity." Biochim.Biophys.Acta.1001. 185-190 (1989)

Kawai, G.：“香菇子实体中脑苷脂的分子种类及其生物活性。”

Hirata, A.: "Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake)." Biol..Pharm.Bull.17. 539-542 (1994)

Hirata, A.：“源自灰树花（舞茸）的蛋白多糖单克隆抗体。”

宮崎由子・佐々木 郁・山本有紀・矢野郁也: "マイタケ由来スフィンゴ糖脂質におけるヒト好中球及び単球の貧食活性" 脂質生化学研究. 39. 151-154 (1997)

Yuko Miyazaki、Ikuru Sasaki、Yuki Yamamoto 和 Ikuya Yano：“来自舞茸蘑菇的鞘糖脂中人类中性粒细胞和单核细胞的下噬活性”脂质生物化学研究 39. 151-154 (1997)。