Effects on the immune response and inhibition of acetylcholine esterase activity by Polygonum hydropiper extracts.

本文报道了加热的虎杖提取物(Ayutade和Benitade：Tade)对人细胞免疫反应的影响和对乙酰胆碱酯酶(AChE)的抑制作用。(1)Tade以剂量依赖的方式促进中性粒细胞和单核细胞的吞噬活性和吞噬小体-溶酶体融合。由于TADE不影响中性粒细胞释放超氧阴离子，TADE组分可能只影响中性粒细胞的某些功能。(2)用Ellman法定量测定了Ayutade和Benitade对AChE的抑制程度。Tade的两种成分均可抑制AChE活性。贝尼塔德的水提取物和乙酸乙酯提取物对AChE的抑制活性尤为显著。(3)对含有TADE成分的饮食菜单进行了干预试验(10名健康人)。比较膳食摄入TADE与对照膳食摄入对认知功能的影响，TADE可能有助于改善和预防痴呆。

We describe the effects of heated Polygonum hydropiper extracts(Ayutade and Benitade:TADE)on the immune response of human cells and inhibition of acetylcholine esterase(AChE).(1)TADE increased the phagocytic activity and phagosome-lysosome fusion of neutrophils and monocytes in a dose-dependent manner. The TADE components may affect only certain functions of human neutrophils because TADE did not affect superoxide anion release from neutrophils.(2)The degree of inhibition of AChE by Ayutade and Benitade was measured quantitatively with the Ellman method. Both components of TADE inhibited AChE activity. The AChE inhibitory activity of the water extract and ethyl acetate extract of Benitade was especially remarkable.(3)An intervention examination(10 healthy persons) of a dietary menu containing TADE components was carried out. The effects of dietary intake of TADE on cognitive function were compared with control dietary intake.TADE may be useful for improving and preventing dementia.