Analyses of molecular mechanism for the host specificity determination in nucleopolyhedroviruses

核多角体病毒宿主特异性测定的分子机制分析

基本信息

批准号：
10306005
负责人：
KOBAYASHI Michihiro
金额：
$ 25.34万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

A number of clonal isolates of nucleopolyhedrovirus (NPV) were derived from uncloned population from the larvae of Spodoptera litura (SpltMNPV) and S. exigua (SeMNPV) collected in the field. These NPV isolates together with NPVs from Autographa californica (AcMNPV), Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Orgyia pseudotsugata (OpMNPV), and Lymantria dispar (LdMNPV) were characterized for their biological properties in seven different cell lines derived from S. frugiperda (Sf9), S. litura (SpLi), S. littoralis (CLS-79), S. exigua (Se301), L dispar (Ld652Y), Spilosoma imparilis (Splm), and B. mori (BmN-4), On the basis of the data oh viral DNA replication, viral structural protein synthesis, budded virion yield, polyhedrin synthesis, and polyhedral formation, it was found that each of these NPV isolates established specific interactions with the respective cell lines. These interactions included varying types of abortive infection and apoptosis as well as productive infection. To provide insights into the molecular mechanisms underlying the host specificity determination of NPVs, a number viral genes including gp64, ie-1 (immediate early gene-1), vlf-1(very late factor-1), iaps (inhibitors of apoptosis), p35, hrs (homologous repeated regions), pcna (proliferating cell nuclear antigen), and egt (ecdysone UDP-glucosyltransterase) were identified and characterized. Functional analyzes suggested that some of these viral genes were relevant to the infectious abortion and apoptosis in the cells infected with NPVs.

核植物学病毒（NPV）的许多克隆分离株源自从孢子虫litura的幼虫（Spltmnpv）和exigua（Semnpv）（SemnPV）的幼虫中。这些NPV分离株与来自加州Autographa（ACMNPV）的NPV，Bombyx Mori（BMNPV），hycunpv杂种（HycunPV），Pseudotsugata（opmnpv）（OPMNPV）和Lymantria dispar（LDMNPV）的七个不同的细胞属性。（SF9），S。Litura（Spli），S。littoralis（Cls-79），S。exigua（SE301），L dispar（ld652y），基于数据OH病毒DNA复制，病毒式蛋白质，合成的potter anter anter，s spilosoma incrialis（splm）和B. mori（splm）和B. mori（bmn-4）多面体形成，发现这些NPV分离株中的每一个都与各自的细胞系建立了特定的相互作用。这些相互作用包括不同类型的流入感染和凋亡以及生产性感染。 To provide insights into the molecular mechanisms underlying the host specificity determination of NPVs, a number viral genes including gp64, ie-1 (immediate early gene-1), vlf-1(very late factor-1), iaps (inhibitors of apoptosis), p35, hrs (homologous repeated regions), pcna (proliferating cell nuclear antigen), and egt (ecdysone鉴定并表征了UDP-葡萄糖基细胞酶）。功能分析表明，其中一些病毒基因与感染了NPV的细胞中的传染性流产和凋亡有关。