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Analyses of molecular mechanism for the host specificity determination in nucleopolyhedroviruses

核多角体病毒宿主特异性测定的分子机制分析

基本信息

批准号：
10306005
负责人：
KOBAYASHI Michihiro
金额：
$ 25.34万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

A number of clonal isolates of nucleopolyhedrovirus (NPV) were derived from uncloned population from the larvae of Spodoptera litura (SpltMNPV) and S. exigua (SeMNPV) collected in the field. These NPV isolates together with NPVs from Autographa californica (AcMNPV), Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Orgyia pseudotsugata (OpMNPV), and Lymantria dispar (LdMNPV) were characterized for their biological properties in seven different cell lines derived from S. frugiperda (Sf9), S. litura (SpLi), S. littoralis (CLS-79), S. exigua (Se301), L dispar (Ld652Y), Spilosoma imparilis (Splm), and B. mori (BmN-4), On the basis of the data oh viral DNA replication, viral structural protein synthesis, budded virion yield, polyhedrin synthesis, and polyhedral formation, it was found that each of these NPV isolates established specific interactions with the respective cell lines. These interactions included varying types of abortive infection and apoptosis as well as productive infection. To provide insights into the molecular mechanisms underlying the host specificity determination of NPVs, a number viral genes including gp64, ie-1 (immediate early gene-1), vlf-1(very late factor-1), iaps (inhibitors of apoptosis), p35, hrs (homologous repeated regions), pcna (proliferating cell nuclear antigen), and egt (ecdysone UDP-glucosyltransterase) were identified and characterized. Functional analyzes suggested that some of these viral genes were relevant to the infectious abortion and apoptosis in the cells infected with NPVs.

从野外采集的斜纹夜蛾（SpltMNPV）和黑斑夜蛾（SeMNPV）幼虫的非克隆种群中分离得到了大量核多角体病毒（NPV）克隆株。这些NPV分离株与加利福尼亚自拟家蚕（AcMNPV）、家蚕（BmNPV）、加利福尼亚棘球绦虫（HycuNPV）、假杉树orgia pseudosugata （OpMNPV）和发散Lymantria dispar （LdMNPV）分离株的NPV分离株进行了生物学特性的鉴定，鉴定菌株分别为frugiperda （Sf9）、S. litura （SpLi）、S. littoralis （CLS-79）、S. exigua （Se301）、L dispar （Ld652Y）、Spilosoma imparis （Splm）和B. mori （BmN-4）。通过对病毒结构蛋白合成、出芽病毒粒子产量、多面蛋白合成和多面体形成的分析，发现每一种NPV分离株都与各自的细胞系建立了特异性相互作用。这些相互作用包括不同类型的流产感染和细胞凋亡以及生产性感染。为了深入了解npv宿主特异性决定的分子机制，我们鉴定并表征了许多病毒基因，包括gp64、ie-1（立即早期基因-1）、vlf-1（非常晚期因子-1）、iaps（凋亡抑制剂）、p35、hrs（同源重复区）、pcna（增殖细胞核抗原）和egt（脱皮激素udp -葡萄糖基转移酶）。功能分析表明，其中部分病毒基因与npv感染细胞的感染性流产和凋亡有关。