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Analyses of molecular mechanism for the host specificity determination in nucleopolyhedroviruses

核多角体病毒宿主特异性测定的分子机制分析

基本信息

批准号：
10306005
负责人：
KOBAYASHI Michihiro
金额：
$ 25.34万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

A number of clonal isolates of nucleopolyhedrovirus (NPV) were derived from uncloned population from the larvae of Spodoptera litura (SpltMNPV) and S. exigua (SeMNPV) collected in the field. These NPV isolates together with NPVs from Autographa californica (AcMNPV), Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Orgyia pseudotsugata (OpMNPV), and Lymantria dispar (LdMNPV) were characterized for their biological properties in seven different cell lines derived from S. frugiperda (Sf9), S. litura (SpLi), S. littoralis (CLS-79), S. exigua (Se301), L dispar (Ld652Y), Spilosoma imparilis (Splm), and B. mori (BmN-4), On the basis of the data oh viral DNA replication, viral structural protein synthesis, budded virion yield, polyhedrin synthesis, and polyhedral formation, it was found that each of these NPV isolates established specific interactions with the respective cell lines. These interactions included varying types of abortive infection and apoptosis as well as productive infection. To provide insights into the molecular mechanisms underlying the host specificity determination of NPVs, a number viral genes including gp64, ie-1 (immediate early gene-1), vlf-1(very late factor-1), iaps (inhibitors of apoptosis), p35, hrs (homologous repeated regions), pcna (proliferating cell nuclear antigen), and egt (ecdysone UDP-glucosyltransterase) were identified and characterized. Functional analyzes suggested that some of these viral genes were relevant to the infectious abortion and apoptosis in the cells infected with NPVs.

许多核多角体病毒 (NPV) 克隆分离株来源于现场收集的斜纹夜蛾 (SpltMNPV) 和甜菜夜蛾 (SeMNPV) 幼虫的非克隆群体。这些 NPV 分离株以及来自苜蓿银纹夜蛾 (AcMNPV)、家蚕 (BmNPV)、美国白蛾 (HycuNPV)、伪蛆 (OpMNPV) 和舞毒蛾 (LdMNPV) 的 NPV 在来自草地贪夜蛾的七种不同细胞系中的生物学特性进行了表征。 (Sf9)、S. litura (SpLi)、S. littoralis (CLS-79)、S. exigua (Se301)、Ld652Y)、Spilosoma imparilis (Splm) 和 B. mori (BmN-4)，基于病毒 DNA 复制、病毒结构蛋白合成、出芽病毒颗粒产量、多角体蛋白合成和多面体形成后，发现这些 NPV 分离株中的每一种都与各自的细胞系建立了特定的相互作用。这些相互作用包括不同类型的流产感染和细胞凋亡以及生产性感染。为了深入了解 NPV 宿主特异性确定的分子机制，许多病毒基因包括 gp64、ie-1（立即早期基因-1）、vlf-1（非常晚期因子-1）、iaps（凋亡抑制剂）、p35、hrs（同源重复区域）、pcna（增殖细胞核抗原）和 egt（蜕皮激素） UDP-葡萄糖基转移酶）被鉴定和表征。功能分析表明，其中一些病毒基因与感染 NPV 的细胞中的感染性流产和细胞凋亡有关。