Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration : basic and clinical research

目的。目的：建立自体虹膜色素上皮细胞移植治疗老年性黄斑变性。我们调查了移栽后的成活率。猴眼中的IPE细胞。通过周边虹膜切除术获得3只猴的自体IPE细胞，并用自体血清进行细胞培养。培养的细胞用DiI标记，每只猴经玻璃体入路进行黄斑下区移植。每月进行眼底检查、摄影和荧光素血管造影术。移植后6个月行组织化学分析和电子显微镜检查。Auto IPE细胞在自体血清中生长良好。黄斑下移植后未发现荧光素渗漏或视网膜增厚。我们在移植后6个月的视网膜组织学和电子显微镜下观察到自体IPE细胞的存在，并证明移植的细胞比宿主视网膜色素上皮细胞着色更少，绒毛膜更少。然而，一些移植的细胞看起来像是拥抱了光感受器的外节。用自身血清建立猕猴自体IPE细胞培养模型。培养的IPE细胞移植6个月后，仍可观察到该区域的细胞。这种方法可能在需要自体细胞移植以防止宿主移植排斥反应的人类需求中具有潜在的用途，并提供一种类似视网膜色素上皮功能的替代物质

Purpose. To establish autologous iris pigment epitheial cell transplantation in patients with age related macular degeneration. We investgated the survival of transplanted. IPE cells in monkey eye.Methods. Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cells were performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by transvitreal approach to submacular region of each monkey. Fundus examination, photography, and fluorescein angiography were performed monthly. Histochemical analysis and electron microscopy were also performed six monthsafter transplantation Results. Auto IPE cells grew well in auto serum. No fluorescein leakage or retinal thickening was observed after submacular transplantation.We demonstrated the presence of autologous IPE cells in the resion 6months after transplantation by histlogic and electron microscopy also demonstrated that the transplanted cells appeared to be less pigmented and to have less mitchondoria than did the host retinal pigment epithelial cells. However, some transplanted cells looked as if they embrace the photoreceptor outer segments.Discussion. Autologous IPE cell culture of the monkey was established using auto serum. Six months after transplantaiton of of cultured IPE cells, we could still observe the cells in region. This method may have potential use in human desiese that reqires autologous cell transplantation to prevent host-graft rejection, and to provide an alternative substance that functions like retinal pigment epithelium