Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration : basic and clinical research

目的。目的：建立自体虹膜色素上皮细胞移植治疗老年性黄斑变性的临床疗效。我们调查了移植后的存活情况。猴眼IPE细胞。通过周围虹膜切除术获得3只猴子的自体IPE细胞，并用自体血清进行细胞处理。培养细胞用DiI标记，经玻璃体入路移植到每只猴的黄斑下区。每月进行眼底检查、摄影和荧光素血管造影。移植6个月后进行组织化学分析和电镜观察。自体IPE细胞在自体血清中生长良好。黄斑下移植术后未见荧光素渗漏或视网膜增厚。我们在移植后6个月通过组织学和电子显微镜证明了自体IPE细胞的存在，移植细胞的色素和线粒体比宿主视网膜色素上皮细胞少。然而，一些移植的细胞看起来好像包围了感光器的外节。采用自体血清建立猴IPE细胞培养。培养的IPE细胞移植6个月后，仍能在局部观察到细胞的变化。这种方法可能在需要自体细胞移植以防止宿主-移植物排斥的人类疾病中有潜在的应用，并提供一种功能类似视网膜色素上皮的替代物质

Purpose. To establish autologous iris pigment epitheial cell transplantation in patients with age related macular degeneration. We investgated the survival of transplanted. IPE cells in monkey eye.Methods. Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cells were performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by transvitreal approach to submacular region of each monkey. Fundus examination, photography, and fluorescein angiography were performed monthly. Histochemical analysis and electron microscopy were also performed six monthsafter transplantation Results. Auto IPE cells grew well in auto serum. No fluorescein leakage or retinal thickening was observed after submacular transplantation.We demonstrated the presence of autologous IPE cells in the resion 6months after transplantation by histlogic and electron microscopy also demonstrated that the transplanted cells appeared to be less pigmented and to have less mitchondoria than did the host retinal pigment epithelial cells. However, some transplanted cells looked as if they embrace the photoreceptor outer segments.Discussion. Autologous IPE cell culture of the monkey was established using auto serum. Six months after transplantaiton of of cultured IPE cells, we could still observe the cells in region. This method may have potential use in human desiese that reqires autologous cell transplantation to prevent host-graft rejection, and to provide an alternative substance that functions like retinal pigment epithelium