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Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration : basic and clinical research

Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration : basic and clinical research
自体虹膜色素上皮细胞移植治疗年龄相关性黄斑变性:基础和临床研究
批准号:
10307041
负责人:
TAMAI Makoto
金额:
$23.94万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 2000

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中文摘要
翻译
目的。目的:建立自体虹膜色素上皮细胞移植治疗老年性黄斑变性。我们调查了移栽后的成活率。猴眼中的IPE细胞。通过周边虹膜切除术获得3只猴的自体IPE细胞,并用自体血清进行细胞培养。培养的细胞用DiI标记,每只猴经玻璃体入路进行黄斑下区移植。每月进行眼底检查、摄影和荧光素血管造影术。移植后6个月行组织化学分析和电子显微镜检查。Auto IPE细胞在自体血清中生长良好。黄斑下移植后未发现荧光素渗漏或视网膜增厚。我们在移植后6个月的视网膜组织学和电子显微镜下观察到自体IPE细胞的存在,并证明移植的细胞比宿主视网膜色素上皮细胞着色更少,绒毛膜更少。然而,一些移植的细胞看起来像是拥抱了光感受器的外节。用自身血清建立猕猴自体IPE细胞培养模型。培养的IPE细胞移植6个月后,仍可观察到该区域的细胞。这种方法可能在需要自体细胞移植以防止宿主移植排斥反应的人类需求中具有潜在的用途,并提供一种类似视网膜色素上皮功能的替代物质
英文摘要
Purpose. To establish autologous iris pigment epitheial cell transplantation in patients with age related macular degeneration. We investgated the survival of transplanted. IPE cells in monkey eye.Methods. Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cells were performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by transvitreal approach to submacular region of each monkey. Fundus examination, photography, and fluorescein angiography were performed monthly. Histochemical analysis and electron microscopy were also performed six monthsafter transplantation Results. Auto IPE cells grew well in auto serum. No fluorescein leakage or retinal thickening was observed after submacular transplantation.We demonstrated the presence of autologous IPE cells in the resion 6months after transplantation by histlogic and electron microscopy also demonstrated that the transplanted cells appeared to be less pigmented and to have less mitchondoria than did the host retinal pigment epithelial cells. However, some transplanted cells looked as if they embrace the photoreceptor outer segments.Discussion. Autologous IPE cell culture of the monkey was established using auto serum. Six months after transplantaiton of of cultured IPE cells, we could still observe the cells in region. This method may have potential use in human desiese that reqires autologous cell transplantation to prevent host-graft rejection, and to provide an alternative substance that functions like retinal pigment epithelium
期刊论文(23)
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会议论文
Abe, T.: "Cytokine gene expression after subretinal transplantation"Tohoku Journal of Experimental Medicine. 189. 179-189 (1999)
Abe,T.:“视网膜下移植后细胞因子基因表达”东北实验医学杂志。
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发表时间: 2000
期刊: Investigative ophthalmology & visual science
影响因子: 4.4
作者: [Y. Wada;M. Nakazawa;T. Abe;M. Tamai]
通讯作者: Y. Wada;M. Nakazawa;T. Abe;M. Tamai
Increased expression of adrenomedullin mRNA in the tissues of intraocular and orbital tumors.
眼内和眼眶肿瘤组织中肾上腺髓质素 mRNA 的表达增加。
DOI: --
发表时间: 2000
期刊: American Journal of Ophthalmology. 129
影响因子: --
作者: [Udono T, Totsune K, Takahashi K, et al.]
通讯作者: et al.
共 18 条
    Establishment of a method for restoring vision using light-gated ion channels and the promotion of optogenetics
    • 批准号:
      21200022
    • 项目类别:
      Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
    • 资助金额:
      $19.8万
    • 财政年份:
      2009
    • 负责人:
      TAMAI Makoto
    • 依托单位:
    Evaluation of protective and adverse effects of gene transferred IPE cell transplantation on degenerative retinal diseases
    • 批准号:
      15390524
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $8.19万
    • 财政年份:
      2003
    • 负责人:
      TAMAI Makoto
    • 依托单位:
    TREATMENT OF RETINAL DEGENERATIVE DISIESE BY TRANSPLANTATION OF GENE TRANSFERRED CELLS
    • 批准号:
      12357010
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $27.79万
    • 财政年份:
      2000
    • 负责人:
      TAMAI Makoto
    • 依托单位:
    Treatment of ischemic retinal injury by administration of various cytokines through vitreous cavity.
    • 批准号:
      07557109
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $11.26万
    • 财政年份:
      1995
    • 负责人:
      TAMAI Makoto
    • 依托单位:
    海外基金