Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration : basic and clinical research

目的.目的建立自体虹膜色素上皮细胞移植治疗老年性黄斑变性的方法。我们研究了移植的存活情况。猴眼IPE细胞。通过外周虹膜切除术获得来自三只猴的自体IPE细胞，并且用自体血清进行细胞培养。用DiI标记培养的细胞，并通过经玻璃体途径对每只猴的黄斑下区域进行移植。每月进行眼底检查、照相和荧光素血管造影。移植后6个月还进行了组织化学分析和电子显微镜检查。自体IPE细胞在自体血清中生长良好。移植后6个月的视网膜组织学和电镜观察显示移植的IPE细胞与宿主视网膜色素上皮细胞相比，色素沉着和线粒体减少。然而，一些移植的细胞看起来好像他们拥抱感光细胞外节。用自体血清建立猴自体IPE细胞培养。培养的IPE细胞移植后6个月，仍能观察到局部细胞。这种方法可能在需要自体细胞移植以防止宿主移植物排斥的人类疾病中具有潜在的用途，并提供一种替代物质，其功能类似于视网膜色素上皮细胞

Purpose. To establish autologous iris pigment epitheial cell transplantation in patients with age related macular degeneration. We investgated the survival of transplanted. IPE cells in monkey eye.Methods. Autologous IPE cells from three monkeys were obtained by peripheral iridectomy, and cells were performed with autologous serum. The cultured cells were labeled with DiI, and transplantation was performed by transvitreal approach to submacular region of each monkey. Fundus examination, photography, and fluorescein angiography were performed monthly. Histochemical analysis and electron microscopy were also performed six monthsafter transplantation Results. Auto IPE cells grew well in auto serum. No fluorescein leakage or retinal thickening was observed after submacular transplantation.We demonstrated the presence of autologous IPE cells in the resion 6months after transplantation by histlogic and electron microscopy also demonstrated that the transplanted cells appeared to be less pigmented and to have less mitchondoria than did the host retinal pigment epithelial cells. However, some transplanted cells looked as if they embrace the photoreceptor outer segments.Discussion. Autologous IPE cell culture of the monkey was established using auto serum. Six months after transplantaiton of of cultured IPE cells, we could still observe the cells in region. This method may have potential use in human desiese that reqires autologous cell transplantation to prevent host-graft rejection, and to provide an alternative substance that functions like retinal pigment epithelium