Neural mechanisms of trigeminal rhythmical activities

三叉神经节律活动的神经机制

• 批准号: 10307050
• 负责人: MATSUYA Tokuzo
• 金额: $ 16.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10307050/
• 关键词: trigeminal; NMDA; rhythmical jaw movement; embryonic; patch clamp; 三叉神経; 運動; 脳; 脳幹; Patch Clamp; 膜電位; 神経生理; 咀嚼; パターンジェネレータ; 脳幹培養

We analyzed the trigeminal neural circuit related to rhythmical jaw movements.We used neonatal rats. We used in vitro isolated brainstem block preparation and whole cell patch clamp technique. Results as follows1. We found rhythmical EPSP on membrane potentials of trigeminal motoneurons, which were thought to be originated from rhythm generators.2. N-methyl D-aspartate (NMDA) receptor agonist, NMA application induced those EPSPs. NMDA receptor must be involved in the rhythm generation circuit.3. In embryonic stages, trigeminal rhyrhmical activities were generated in E-20. In E-18,19, the rhythmical activities were incompletely recognized. However, rhythmical EPSPs were regularly recognized. In E-16,17, the rhythmical activities were not generated. It is likely that the rhythm generation circuit is developed at E-18,19.

我们分析了与下颌节律运动相关的三叉神经回路。采用离体脑干神经阻滞制备和全细胞膜片钳技术。结果如下1.在三叉神经运动神经元的膜电位上发现了节律性EPSP，推测其起源于节律发生器. N-甲基D-天冬氨酸（NMDA）受体激动剂，NMA应用诱导这些EPSP。NMDA受体参与节律产生回路.在胚胎期，三叉神经节律活动产生于E-20。在E-18、19，节律活动不完全被识别。然而，有节奏的EPSPs定期确认。E-16、E-17无节律活动。很可能在E-18，19时形成节律生成回路。