Analysis of Neural Network regarding Oral Dysfunction

口腔功能障碍的神经网络分析

• 批准号: 11671986
• 负责人: MATSUYA Tokuzo
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671986/
• 关键词: Trigeminal Nerve; Rhythm; Mastication; Brainstem; Oral; Neural Network; EPSP; Membrane Potential; ニューラルネットワーク; モトニューロン; プラキシズム

We used the neonatal rats. We made the brainstem block preparation in vitro. We applied GABA receptor antagonist, Bicuculine and the excitatory amino acid, NMDA to induce the rhythmical trigeminal activities. We recorded the membrane potentials of trigeminal motoneuron during rhythmical trigeminal bursting. The series resistant was 60.9±6.8MΩ, whole cell capacitance was 3.5±2.6pF and resting potential was 50.7±6.8mV.After application of Bicuculine and NMDA, firstly EPSP was recorded in the membrane potential. After depolarization, the action potential was enhanced with each EPSP.We concluded that Bicuculine induces depolarization of membrane potentials and that NMDA plays some role in rhythm generation.We also made the brainstem block including respiratory circuit. We made the extracelluler recording from trigeminal nerve, trigeminal motor nucleus and hypoglossal nerve. After recording respiratory activities, we applied Bicuculine and NMDA to the chamber to induce the rhythmical trigeminal activities. After enhancement of the rhythmical trigeminal activities, the respiratory activities decreased. It is likely that there is an antagonistic relationship between these two circuits.In the motor activities such as mastication and respiration, an isolated brain stem block preparation is very useful to analyze the neural network. We will continue the analysis of these neural circuits using this preparation.

我们使用了新生大鼠。我们在体外制备了脑干阻滞制剂。应用GABA受体拮抗剂双库库碱和兴奋性氨基酸NMDA诱导三叉神经节律性活动。我们记录了三叉神经节律性破裂时运动神经元的膜电位。串联电阻为60.9±6.8MΩ，全电池电容为3.5±2.6pF，静息电位为50.7±6.8mV。应用双库林和NMDA后，首先在膜电位中记录EPSP。去极化后，每个EPSP均增强动作电位。我们得出结论，双库碱诱导膜电位去极化，NMDA在节律产生中起一定作用。我们还制作了包括呼吸回路在内的脑干阻滞。我们对三叉神经、三叉运动核和舌下神经进行了细胞外记录。记录呼吸活动后，应用双库库林和NMDA诱导三叉神经节律性活动。节律性三叉神经活动增强后，呼吸活动减弱。这两个回路之间很可能存在一种拮抗关系。在咀嚼和呼吸等运动活动中，孤立的脑干块制备对神经网络分析非常有用。我们将继续使用这种制备方法分析这些神经回路。