Extensive research on D-amino acids present in mammals

Toyo'oka将他开发的一种方法应用于食品和生物样品中D，L-氨基酸的测定。Nagata从Pyrobaculum islandicum分离D-丙氨酸脱氢酶。Misono克隆了谷氨酸消旋酶并测定了其序列。Asano从O. anthropi，并研究了其与功能的关系。江崎分析了从酿酒酵母和粟酒裂殖酵母中获得的氨基酸酶基因。Horiike证明了D-丝氨酸和D-氨基酸氧化酶在哺乳动物大脑中的反向定位。Ymada认为，膜二肽酶可能有助于肾脏疾病的诊断。桥本提出了一个可能的摄取D-丝氨酸进入细胞的刺激去极化。Nishikawa提出内源性D-丝氨酸可能与NMDA甘氨酸结合位点以外的另一个位点结合。Imai证明了谷氨酸转运蛋白的出现在内源性D-天冬氨酸存在的同一位点。香川分离出一种含D-天冬氨酸肽的特异性降解酶。Iwatsubo鉴定了早老素的一个重要功能结构域，作为最C末端的氨基酸，用于增强A β 42的产生。Fujii提出了α A-胆甾醇中天冬氨酸逆反应的立体特异性结构。

Toyo'oka applied a method a method he developed to the determination of D, L-amino acids in food and biosamples. Nagata isolated D-alanine dehydrogenase from Pyrobaculum islandicum. Misono cloned glutamate racemase and determined the sequence of the enzyme. Asano cloned D-amino acid amide specific amidase from O. anthropi and investigated its relation to the function. Esaki analyzed the gene of amino acid rasemase obtained from Saccharomyces cerevisiae and Schizosaccharomyces pombe. Horiike demonstrated a reverse localization of D-serine and D-amino acid oxidase in the mammals brain. Ymada suggested that a membrane dipeptidase might be useful for the diagnosis of kidney disease. Hashimoto suggested a probable uptake of D-serine into a cell by a stimulation of depolarization. Nishikawa proposed a possible binding of endogenous D-serine with an another site in addition to NMDA glycine binding site. Imai demonstrated the emergence of glutamate transporter at the same site of existence of endogenous D-aspartate. Kagawa isolated a specific degradation enzyme of a D-aspartate containing peptide. Iwatsubo Identified an essential functional domain of presenilin as the most C-terminal amino acid for the enhancement of A β 42 production. Fujii proposed a stereospecific structure for the reverse reaction of aspartate in α A-chrystallin.