Study on control method of the nature and concentration of aluminum in surface and subsurface water using silica and microbes

本研究旨在发展地表水和地下水中铝的性质和浓度控制方法。通过野外和实验研究了铝与二氧化硅或微生物的反应机理，得到了以下结果：1.铝与二氧化硅或微生物的反应机理。本文主要研究内容如下：1.研究了土壤水中铝的重要固持材料--拟沸石的形成特性。从黄石国家公园热水形成的硅质沉积物中发现细菌rDNA克隆，其中以西乌霉属和糖单孢属为主。从硅质沉积物中还检测到6配位铝。从Theumus sp.中检测到硅胶诱导蛋白(Sip)。TMY在含硅量超过400ppm的培养基中培养。西乌姆斯菌的细胞生长情况。TMY在含铝的介质中受到抑制，尤其是在200ppm以上。然而，在100ppm铝环境中获得的培养物表现出耐碱性。添加二氧化硅对细胞生长有促进作用。为阐明铝离子在微生物表面的吸附机理，研究了阳离子交换树脂和螯合树脂对铝的吸附行为。核磁共振数据表明，四配位铝随硅铝比的增加而增加。

This study was planned to develop the control method of the nature and concentration of aluminum in surface and subsurface waters. Reaction mechanisms between aluminum and silica or microbes were studied through field and experimental works and the following results were obtained.1. Formation characteristics of imogolite, which is important materials in fixation of aluminum from soil water, were studied.2. The bacterial rDNA clones were found from siliceous deposits formed from hot waters in Yellowstone National Park and the genera Theumus and Saccharomonospora were dominated. Also 6-coodinated aluminum was detected from the siliceous deposit.3. Silica-induced protein (Sip) was detected from Theumus sp. TMY cultivated in medium containing over 400 ppm of silica.4. The cell growth of Theumus sp. TMY was inhibited in medium containing aluminum especially over 200 ppm. However, the culture obtained from 100 ppm of aluminum environment showed the Altolerance. The cell growth was promoted with silica addition.5. To elucidate the uptake mechanism of aluminum ions on the surface of microbes, the adsorption behavior of aluminum on cation exchange resin and chelate resin was studied. NMR data shows the increasing 4-coordinated aluminum with increasing silica/alumina ratio.