Study on control method of the nature and concentration of aluminum in surface and subsurface water using silica and microbes

本研究旨在开发地表水和地下沃茨中铝的性质和浓度的控制方法。通过现场和室内试验，研究了铝与硅、微生物的反应机理，取得了以下成果.研究了土壤水铝固定的重要物质--伊毛沸石的形成特征.从黄石国家公园的热水沃茨形成的硅质沉积物中发现了细菌rDNA克隆，以Theumus属和Pneumononospora属为主。硅质沉积物中还检测到6配位铝.在含硅量超过400 ppm的培养基中培养的Theumus sp. TMY中检测到硅诱导蛋白（Sip）.在含铝量超过200 ppm的培养基中，细胞生长受到抑制。然而，从100 ppm的铝环境中获得的培养物显示出耐铝性。二氧化硅的加入促进了细胞的生长.为阐明微生物对铝离子的吸附机理，研究了铝离子在阳离子交换树脂和螯合树脂上的吸附行为。NMR数据显示随着二氧化硅/氧化铝比率的增加，4-配位铝增加。

This study was planned to develop the control method of the nature and concentration of aluminum in surface and subsurface waters. Reaction mechanisms between aluminum and silica or microbes were studied through field and experimental works and the following results were obtained.1. Formation characteristics of imogolite, which is important materials in fixation of aluminum from soil water, were studied.2. The bacterial rDNA clones were found from siliceous deposits formed from hot waters in Yellowstone National Park and the genera Theumus and Saccharomonospora were dominated. Also 6-coodinated aluminum was detected from the siliceous deposit.3. Silica-induced protein (Sip) was detected from Theumus sp. TMY cultivated in medium containing over 400 ppm of silica.4. The cell growth of Theumus sp. TMY was inhibited in medium containing aluminum especially over 200 ppm. However, the culture obtained from 100 ppm of aluminum environment showed the Altolerance. The cell growth was promoted with silica addition.5. To elucidate the uptake mechanism of aluminum ions on the surface of microbes, the adsorption behavior of aluminum on cation exchange resin and chelate resin was studied. NMR data shows the increasing 4-coordinated aluminum with increasing silica/alumina ratio.