Breeding of low prolamin rice

点击翻译按钮获取中文摘要

Screening of low prolamin mutantsLow prolamin mutant lines were selected from the progeny of the mutagenesis treatment. The diverse variation for prolamin polypeptides were detected from the native rice varieties, about 2000, collected from some countries. The crossing among low prolamin varieties was done, the progeny is being analyzed.Brewing prosperity of low prolamin mutantThe low prolamin mutant, esp2, showed the low acid degree and low amino acid degree by small scale brewing test, though the mutant showed very high dissolving degree. The results will be helpful on the efficient utilization of the materials.Isolation of the gene for low prolaminBy the results of the linkage analysis of the low prolamin mutant, esp1 gene is positioned within the 1.4 cM region on chromosome 4. The high-density linkage map of esp1 gene will be constructed.The genes of PDI and BiP, related to the accumulation of the prolamin into PBI, were cloned using the cDNA library constructed from the developing seed of rice cultivar 'Kinmaze'. The specific antibodies were prepared based on the cDNA clone.Cytochemical analysis of the low prolamin mutantsProlamin consists of two classes, the cysteine residue poor prolamin (Cys-P prolamin) and the cysteine residue rich prolamin (Cys-R prolamin). The analysis of low prolamin mutants esp3 and Esp4 indicates that Cys-R and Cys-P prolamins are accumulated into PBI at the early and late stages, respectively, during seed development. The analysis by the antibodies to each prolamin classes indicates that Cys-P and Cys-R prolamins accumulate in the center and the periphery of PBI, respectively. It is thought that Cys-P prolamin plays an important role as a core and Cys-R plays the role of filling the inside of PBI.