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Breeding of low prolamin rice

低谷醇蛋白水稻的选育

基本信息

批准号：
10356001
负责人：
SATOH Hikaru
金额：
$ 18.01万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10356001/
关键词：
Rice Brewing prosperity prolamin Linkage Analysis Mutation Protein Body cDNA cloning 胚乳貯蔵タンパク質突然変異体 cDNAライブラリー SDS-PAGE 遺伝分析 MNU受精卵処理旺気貯蔵タンパク質

项目摘要

Screening of low prolamin mutantsLow prolamin mutant lines were selected from the progeny of the mutagenesis treatment. The diverse variation for prolamin polypeptides were detected from the native rice varieties, about 2000, collected from some countries. The crossing among low prolamin varieties was done, the progeny is being analyzed.Brewing prosperity of low prolamin mutantThe low prolamin mutant, esp2, showed the low acid degree and low amino acid degree by small scale brewing test, though the mutant showed very high dissolving degree. The results will be helpful on the efficient utilization of the materials.Isolation of the gene for low prolaminBy the results of the linkage analysis of the low prolamin mutant, esp1 gene is positioned within the 1.4 cM region on chromosome 4. The high-density linkage map of esp1 gene will be constructed.The genes of PDI and BiP, related to the accumulation of the prolamin into PBI, were cloned using the cDNA library constructed from the developing seed of rice cultivar 'Kinmaze'. The specific antibodies were prepared based on the cDNA clone.Cytochemical analysis of the low prolamin mutantsProlamin consists of two classes, the cysteine residue poor prolamin (Cys-P prolamin) and the cysteine residue rich prolamin (Cys-R prolamin). The analysis of low prolamin mutants esp3 and Esp4 indicates that Cys-R and Cys-P prolamins are accumulated into PBI at the early and late stages, respectively, during seed development. The analysis by the antibodies to each prolamin classes indicates that Cys-P and Cys-R prolamins accumulate in the center and the periphery of PBI, respectively. It is thought that Cys-P prolamin plays an important role as a core and Cys-R plays the role of filling the inside of PBI.

低醇溶蛋白突变体的筛选从诱变处理的后代中筛选出低醇溶蛋白突变体系。从一些国家收集的约2000个水稻地方品种中检测到醇溶蛋白多肽的多样性变异。低醇溶蛋白突变体esp 2的溶出度很高，但经小规模酿造试验，其酸度和氨基酸含量均较低。低醇溶蛋白基因的分离通过对低醇溶蛋白突变体的连锁分析，将esp 1基因定位于第4染色体上的1.4cM区域。利用构建的水稻品种“金玛泽”发育种子cDNA文库，克隆了与醇溶蛋白积累成PBI相关的基因PDI和BiP。低醇溶谷蛋白突变体的细胞化学分析醇溶谷蛋白分为贫半胱氨酸残基的醇溶谷蛋白（Cys-P prolamin）和富半胱氨酸残基的醇溶谷蛋白（Cys-R prolamin）。低醇溶蛋白突变体esp 3和Esp 4的分析表明，Cys-R和Cys-P醇溶蛋白在种子发育的早期和后期分别积累成PBI。通过对每种醇溶谷蛋白类别的抗体的分析表明，Cys-P和Cys-R醇溶谷蛋白分别积累在PBI的中心和外周。认为Cys-P醇溶蛋白在PBI中起着核心作用，Cys-R起着填充PBI内部的作用。