Molecular biological analysis for foregut malformation in a rat model

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Three dimensional image reconstruction od the developing foregut in normal and adriamycin (ADM)-induced tracheoesophageal fistula (TEF) rats.In the nornal embryo, the lung bud appeared just below the pharyngeal foregut on day 11 and it elongated caudally to shape into the trachea as they grew. A 'tracheoesophageal septum' did not emerge. In the TEF embryos, although the lung bud appeared in a similar position on day 11, trachea and esophagus did not separate and only a common foregut tube elongated caudally, then bilateral bronchial primordia emerged directly at the level of bifurcation. furthermore, abnormal development of the notochord was evident, being retarded in separation from the foregut, finally descending along a more ventral course and bending sharply around the level of the bifurcation. The development of TEF was almost complete by day 13.In sunnary, the trachea seemed to be formed only by caudal elingation of the lung bud in the normal embryo. In the ADM-induced TEF embryos, the upper foregut appeared to develop only into the trachea and dthis maldevelopment may be implicated in the abnormal interaction between the foregut and the surrounding mesenchyme.