Simple nervous system approach to the mechanisms of associative learning

联想学习机制的简单神经系统方法

基本信息

批准号：
10480176
负责人：
KIRINO Yutaka
金额：
$ 8.19万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

This work was aimed at clarifying the mechanisms of leaning and memory using physiological and molecular biological methods. First, physiological recordings were made in the procerebrum, which is the olfactory center of the slug, in isolated bran preparations after odor-taste associative conditioning Optical recording of the membrane potential revealed that a cluster of neurons in the procerebrum depolarizes selectively in response to leaned odors.By the mRNA differential display method, we found a gene whose expression elevated specifically after odor-taste associative conditioning, and its sequence has been determined. This gene encoded 121 amino acids, and named LAPS18. To clarify its function, the expression pattern of this gene was determined. The expression of the LAPS18 protein was significantly increased 12-48 h after conditioning compared to the control group. This suggests that the LAPS18 protein is related to the late phase events of learning. Immunohistochemical analysis indicated that LAPS18 protein was present in the entire procerebrum, and localized to the neuropil layers of the procerebrum after conditioning, indicating a conditioning-specific changes in localization. By corUoca1 laser scanning microscopy, the LAPS18 protein was found to be localized to the nerve terminals, especially regions of cell contact. These results suggest that the LAPS18 protein is an extrace11ular matrix protein secreted at nerve terminals and modulate the synaptic connections. The homologue genes of LAPS18 were found in various Vertebrate species. Therefore, LAPS18 was thought to play essential roles in learning and memory.

这项工作旨在使用生理和分子生物学方法阐明倾斜和记忆的机制。首先，在Procerebrum中进行了生理记录，这是Slug的嗅觉中心，在隔离的Bran制剂中，在气味泥浆中的晶状疗程后，对膜潜力的光学记录进行光学记录，在procerebrum中的一群神经元显示出对倾向的气味的响应，我们发现了对倾向的疾病，我们发现了源自偏差的源。并确定了其序列。该基因编码了121个氨基酸，命名为LAPS18。为了阐明其功能，确定了该基因的表达模式。与对照组相比，调节后LAPS18蛋白的表达显着增加了12-48小时。这表明LAPS18蛋白与学习的后期事件有关。免疫组织化学分析表明，调节后的整个procerebrum中存在LAPS18蛋白，并将其定位于Procerebrum的Neuropil层，表明定位的条件特异性变化。通过Coruoca1激光扫描显微镜，发现LAPS18蛋白位于神经末端，尤其是细胞接触区域。这些结果表明，LAPS18蛋白是在神经末端分泌的外推11个蛋白，并调节突触连接。 LAPS18的同源基因在各种脊椎动物中发现。因此，LAPS18被认为在学习和记忆中起着重要的作用。