An Engineered Surface of Mucociliary Transport for Medical Devices

用于医疗器械的粘膜纤毛运输工程表面

• 批准号: 10627572
• 负责人: Yuliang Xie
• 金额: $ 31.9万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627572
• 关键词: 3D Print; Acoustics; Adhesions; Agar; Aluminum Oxide; Animal Model; Antibiotics; Bacteria; Bacterial Infections; Banana; Biological; Biology; Biomedical Engineering; Breathing; Chemistry; Cilia; Clinical; Communities; Development; Devices; Dimensions; Economics; Effectiveness; Engineering; Environment; Epithelium; Family suidae; Female genitalia; Fertilization; Foundations; Friction; Gel; Gold; Health; Human; Hydrogels; Implant; In Vitro; Infection; Inhalation; Iowa; Lateral; Lung; Medical Device; Medicine; Methods; Microfluidics; Modification; Motion; Movement; Mucociliary Clearance; Mucolytics; Mucous body substance; Ocular Prosthesis; Organ; Outcome; Patients; Penetration; Pharmaceutical Preparations; Physiology; Polymers; Polysaccharides; Process; Reporting; Research; Research Personnel; Respiratory System; Risk; Side; Sterility; Structure; Suction; Surface; Technology; Testing; Tissues; Trachea; Tracheostomy Tube; Transportation; Tube; Universities; Validation; Voice Prostheses; animal tissue; care burden; chronic infection; design; driving force; endotracheal; ethylene glycol; fabrication; hydrophilicity; in vivo; in vivo evaluation; infection burden; innovation; mechanical force; mucus clearance; pathogen; poly(ethylene glycol)diacrylate; polydimethylsiloxane; porcine model; prevent; programs; prototype; reproductive tract; side effect; slug; social; sperm cell; stem; success; surface coating; ultrasound; vibration

PROJECT SUMMARY/ABSTRACT Mucus contacted medical devices, such as airway devices and eye prostheses, suffer from mucus accumulation. Plugged mucus causes bacterial infection, airway blockage, and a requirement of frequent device cleaning and replacement, which adds significant care burdens for the patient and support community. Current approaches to mitigate mucus accumulation involve strong mechanical forces or medications, thus having intrinsic limitations and side effects. Mucociliary transport (MCT), a process by which waves of beating cilia move a blanket of mucus, forms the first-line barrier against infection in respiratory and genital tracts. Inspired by the effectiveness of MCT in clearing mucus, the objective of this project is to develop an engineered surface that enables MCT function (i.e., an engineered surface of MCT). Our objective will be achieved through a combination of cilia fabrication, surface modification, and acoustic actuation with the following aims: fabricate engineered surfaces with polarized ciliary structures (Aim 1); understand mucus stickiness on engineered surface of MCT. (Aim 2); develop platforms to test acoustic actuated MCT on engineered surfaces in vitro and in vivo (Aim 3). The proposed research is rationally built on experimental feasibility, investigator expertise, and a supportive research environment. The feasibility is supported by experimental successes in polymer ciliary surfaces, slug and pig models of mucus, and vibration of microstructures with acoustic waves. The team of investigators have expertise in MCT, microfluidics, acoustics, 3D printing, and animal models. The proposed research will be conducted within the environment of the world- renowned Lung Physiology Research Center and Roy J. Carver Department of Biomedical Engineering at the University of Iowa, with broad availability of complementary expertise in biology and engineering. The expected outcomes of this project include revealing the mechanism of MCT on engineered surfaces and delivering an innovative medical device product incorporating an engineered surface of MCT.

项目摘要/摘要 接触粘液的医疗器械，如呼吸道设备和假眼，会患上粘液 积累。粘液堵塞会导致细菌感染、呼吸道堵塞，并要求经常 设备清洗和更换，这增加了患者和支持社区的护理负担。 目前减轻粘液堆积的方法包括强大的机械力或药物，因此 具有内在的局限性和副作用。粘液纤毛运输(MCT)，即一波又一波的搏动 纤毛移动一层粘液，形成抵抗呼吸道和生殖道感染的第一线屏障。 受到MCT在清除粘液方面的有效性的启发，这个项目的目标是开发一种 启用MCT功能的工程表面(即MCT的工程表面)。我们的目标将是 通过纤毛制造、表面改性和声学驱动与 以下目标：制造具有极化纤毛结构的工程化表面(目标1)；了解粘液 MCT工程表面的粘附性。(目标2)；开发测试声控MCT的平台 体外和活体工程表面(目标3)。建议的研究是建立在实验的基础上的。 可行性、研究人员的专业知识和支持性的研究环境。可行性得到以下方面的支持 在聚合物纤毛表面、鼻涕和粘液的猪模型以及振动方面的实验成功 具有声波的微结构。调查团队在MCT、微流体、声学、 3D打印和动物模型。拟议的研究将在世界环境下进行- 著名的肺生理学研究中心和罗伊·J·卡弗生物医学工程系 爱荷华大学，在生物学和工程学方面拥有广泛的互补专业知识。预期中的 该项目的成果包括揭示了工程表面上的MCT机理，并提供了一个 包含MCT工程表面的创新医疗设备产品。