An Engineered Surface of Mucociliary Transport for Medical Devices

用于医疗器械的粘膜纤毛运输工程表面

• 批准号: 10627572
• 负责人: Yuliang Xie
• 金额: $ 31.9万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627572
• 关键词: 3D Print; Acoustics; Adhesions; Agar; Aluminum Oxide; Animal Model; Antibiotics; Bacteria; Bacterial Infections; Banana; Biological; Biology; Biomedical Engineering; Breathing; Chemistry; Cilia; Clinical; Communities; Development; Devices; Dimensions; Economics; Effectiveness; Engineering; Environment; Epithelium; Family suidae; Female genitalia; Fertilization; Foundations; Friction; Gel; Gold; Health; Human; Hydrogels; Implant; In Vitro; Infection; Inhalation; Iowa; Lateral; Lung; Medical Device; Medicine; Methods; Microfluidics; Modification; Motion; Movement; Mucociliary Clearance; Mucolytics; Mucous body substance; Ocular Prosthesis; Organ; Outcome; Patients; Penetration; Pharmaceutical Preparations; Physiology; Polymers; Polysaccharides; Process; Reporting; Research; Research Personnel; Respiratory System; Risk; Side; Sterility; Structure; Suction; Surface; Technology; Testing; Tissues; Trachea; Tracheostomy Tube; Transportation; Tube; Universities; Validation; Voice Prostheses; animal tissue; care burden; chronic infection; design; driving force; endotracheal; ethylene glycol; fabrication; hydrophilicity; in vivo; in vivo evaluation; infection burden; innovation; mechanical force; mucus clearance; pathogen; poly(ethylene glycol)diacrylate; polydimethylsiloxane; porcine model; prevent; programs; prototype; reproductive tract; side effect; slug; social; sperm cell; stem; success; surface coating; ultrasound; vibration

PROJECT SUMMARY/ABSTRACT Mucus contacted medical devices, such as airway devices and eye prostheses, suffer from mucus accumulation. Plugged mucus causes bacterial infection, airway blockage, and a requirement of frequent device cleaning and replacement, which adds significant care burdens for the patient and support community. Current approaches to mitigate mucus accumulation involve strong mechanical forces or medications, thus having intrinsic limitations and side effects. Mucociliary transport (MCT), a process by which waves of beating cilia move a blanket of mucus, forms the first-line barrier against infection in respiratory and genital tracts. Inspired by the effectiveness of MCT in clearing mucus, the objective of this project is to develop an engineered surface that enables MCT function (i.e., an engineered surface of MCT). Our objective will be achieved through a combination of cilia fabrication, surface modification, and acoustic actuation with the following aims: fabricate engineered surfaces with polarized ciliary structures (Aim 1); understand mucus stickiness on engineered surface of MCT. (Aim 2); develop platforms to test acoustic actuated MCT on engineered surfaces in vitro and in vivo (Aim 3). The proposed research is rationally built on experimental feasibility, investigator expertise, and a supportive research environment. The feasibility is supported by experimental successes in polymer ciliary surfaces, slug and pig models of mucus, and vibration of microstructures with acoustic waves. The team of investigators have expertise in MCT, microfluidics, acoustics, 3D printing, and animal models. The proposed research will be conducted within the environment of the world- renowned Lung Physiology Research Center and Roy J. Carver Department of Biomedical Engineering at the University of Iowa, with broad availability of complementary expertise in biology and engineering. The expected outcomes of this project include revealing the mechanism of MCT on engineered surfaces and delivering an innovative medical device product incorporating an engineered surface of MCT.

项目总结/摘要 粘液接触的医疗装置，如气道装置和眼假体，遭受粘液 积累堵塞的粘液会导致细菌感染，气道阻塞，并需要频繁的呼吸。 设备清洁和更换，这给患者和支持社区增加了显著的护理负担。 目前减轻粘液积聚的方法涉及强机械力或药物，因此， 具有固有的局限性和副作用。粘膜纤毛运输（MCT），一个过程， 纤毛移动一层粘液，形成呼吸道和生殖道抵抗感染的第一道屏障。 受MCT在清除粘液方面的有效性的启发，本项目的目标是开发一种 能够实现MCT功能的工程化表面（即，MCT的工程化表面）。我们的目标是 通过纤毛制造、表面改性和声致动的组合来实现， 以下目标：制造具有极化纤毛结构的工程表面（目标1）;了解粘液 MCT工程表面的粘性。(Aim 2）开发平台，以测试声致动MCT， 体外和体内工程表面（目标3）。建议的研究是合理的实验基础上建立的 可行性，研究者的专业知识和支持性的研究环境。可行性得到以下方面的支持： 实验成功的聚合物纤毛表面，鼻涕虫和猪模型的粘液，振动， 微结构与声波。研究人员团队在MCT，微流体，声学， 3D打印和动物模型。拟议的研究将在世界环境中进行- 著名的肺生理学研究中心和罗伊J.卡弗生物医学工程系， 爱荷华州大学，在生物学和工程学方面拥有广泛的互补专业知识。预期 该项目的成果包括揭示工程表面上MCT的机制并提供 采用MCT工程表面的创新医疗器械产品。