An Engineered Surface of Mucociliary Transport for Medical Devices

用于医疗器械的粘膜纤毛运输工程表面

• 批准号: 10627572
• 负责人: Yuliang Xie
• 金额: $ 31.9万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627572
• 关键词: 3D Print; Acoustics; Adhesions; Agar; Aluminum Oxide; Animal Model; Antibiotics; Bacteria; Bacterial Infections; Banana; Biological; Biology; Biomedical Engineering; Breathing; Chemistry; Cilia; Clinical; Communities; Development; Devices; Dimensions; Economics; Effectiveness; Engineering; Environment; Epithelium; Family suidae; Female genitalia; Fertilization; Foundations; Friction; Gel; Gold; Health; Human; Hydrogels; Implant; In Vitro; Infection; Inhalation; Iowa; Lateral; Lung; Medical Device; Medicine; Methods; Microfluidics; Modification; Motion; Movement; Mucociliary Clearance; Mucolytics; Mucous body substance; Ocular Prosthesis; Organ; Outcome; Patients; Penetration; Pharmaceutical Preparations; Physiology; Polymers; Polysaccharides; Process; Reporting; Research; Research Personnel; Respiratory System; Risk; Side; Sterility; Structure; Suction; Surface; Technology; Testing; Tissues; Trachea; Tracheostomy Tube; Transportation; Tube; Universities; Validation; Voice Prostheses; animal tissue; care burden; chronic infection; design; driving force; endotracheal; ethylene glycol; fabrication; hydrophilicity; in vivo; in vivo evaluation; infection burden; innovation; mechanical force; mucus clearance; pathogen; poly(ethylene glycol)diacrylate; polydimethylsiloxane; porcine model; prevent; programs; prototype; reproductive tract; side effect; slug; social; sperm cell; stem; success; surface coating; ultrasound; vibration

PROJECT SUMMARY/ABSTRACT Mucus contacted medical devices, such as airway devices and eye prostheses, suffer from mucus accumulation. Plugged mucus causes bacterial infection, airway blockage, and a requirement of frequent device cleaning and replacement, which adds significant care burdens for the patient and support community. Current approaches to mitigate mucus accumulation involve strong mechanical forces or medications, thus having intrinsic limitations and side effects. Mucociliary transport (MCT), a process by which waves of beating cilia move a blanket of mucus, forms the first-line barrier against infection in respiratory and genital tracts. Inspired by the effectiveness of MCT in clearing mucus, the objective of this project is to develop an engineered surface that enables MCT function (i.e., an engineered surface of MCT). Our objective will be achieved through a combination of cilia fabrication, surface modification, and acoustic actuation with the following aims: fabricate engineered surfaces with polarized ciliary structures (Aim 1); understand mucus stickiness on engineered surface of MCT. (Aim 2); develop platforms to test acoustic actuated MCT on engineered surfaces in vitro and in vivo (Aim 3). The proposed research is rationally built on experimental feasibility, investigator expertise, and a supportive research environment. The feasibility is supported by experimental successes in polymer ciliary surfaces, slug and pig models of mucus, and vibration of microstructures with acoustic waves. The team of investigators have expertise in MCT, microfluidics, acoustics, 3D printing, and animal models. The proposed research will be conducted within the environment of the world- renowned Lung Physiology Research Center and Roy J. Carver Department of Biomedical Engineering at the University of Iowa, with broad availability of complementary expertise in biology and engineering. The expected outcomes of this project include revealing the mechanism of MCT on engineered surfaces and delivering an innovative medical device product incorporating an engineered surface of MCT.

项目摘要/摘要 粘液联系了医疗设备，例如气道设备和眼影，患有粘液 积累。插入粘液会引起细菌感染，气道阻塞和频繁的要求 设备清洁和更换，为患者和支持社区增加了巨大的护理负担。 当前减轻粘液积累的方法涉及强大的机械力或药物，因此 具有内在的局限性和副作用。粘毛转运（MCT），该过程的跳动波 纤毛移动粘液的毯子，在呼吸道和生殖道中形成了针对感染的一线屏障。 受到MCT在清除粘液中的有效性的启发，该项目的目的是开发一个 可以启用MCT功能的工程表面（即MCT的工程表面）。我们的目标将是 通过结合纤毛制造，表面修饰和声学探测与 以下目的：用偏光睫状结构制造工程表面（AIM 1）；了解粘液 MCT工程表面的粘性。 （目标2）;开发平台测试声学驱动的MCT 在体外和体内设计表面（AIM 3）。拟议的研究是基于实验的 可行性，研究者专业知识和支持性研究环境。可行性由 聚合物睫状表面，粘液和猪模型的实验成功，以及振动的振动 具有声波的微观结构。调查人员团队在MCT，微流体，声学，声学， 3D打印和动物模型。拟议的研究将在世界环境中进行 - 著名的肺部生理研究中心和罗伊·J·卡弗（Roy J. Carver）生物医学工程系 爱荷华大学，具有广泛的生物学和工程专业知识。预期 该项目的结果包括揭示MCT在工程表面上的机制并提供 创新的医疗设备产品结合了MCT的工程表面。