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Polymorphism's of mitochondrial DNA and selection of Japanese Black

日本黑毛线粒体DNA多态性与选育

基本信息

批准号：
10556064
负责人：
TSUJI Soichi
金额：
$ 6.14万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10556064/
关键词：
Japanese Black mitochondria D-loop maternal inheritance PCR-SSCP marbling rib eye area ミトコンドリアD-loop

项目摘要

Japanese black is only one domestic animal which produce economically competitive beef with foreign products, because of producing high grade beef. The genes by which the high quality beef being produced are not yet known. Almost of all researches are concentrated their study on the genome DNA from the nuclei, however, we had paid attention on the genome from mitochondria for past few years. Japanese Black are classified into five groups depending on D-loop DNA sequence of mitochondria. We found that a group, type 4, produce high quality beef. 1)To ascertain if this mitochondrial effect is observed in commercial reproductive herd, cows of more than 8,000 were classified into 5 groups by mitochondrial DNA type. The effect of mitochondrial type is not clear because of environmental effects, such as feeding condition, year season, feedlot etc. But in Hyogo prefecture, the mitochondrial effect was only observed in steers of 28month old finishing. If D-loop has some physiological effects on beef production, as the D-loop region does not code any protein, so that mutations other region than D-loop region, which coordinates with D-loop type should be present. We selected two animals from each mitochondrial type and DNA sequence of whole mitochondria genome was determined. We found type 4 specific mutation in 16SrRNA which may affect on protein synthesis in mitochondria.

日本黑牛是世界上唯一一种能生产出具有经济竞争力的优质牛肉的家畜。生产高质量牛肉的基因尚不清楚。几乎所有的研究都集中在细胞核的基因组DNA上，而线粒体基因组的研究则是近几年才开始的。根据线粒体D-loop DNA序列的不同，将日本黑分为5类。我们发现，第4类群体生产高质量的牛肉。1）为了确定在商业繁殖牛群中是否观察到这种线粒体效应，将超过8，000头奶牛按线粒体DNA类型分为5组。由于饲养条件、季节、饲养场等环境因素的影响，线粒体类型的影响尚不清楚，但在兵库县，线粒体效应仅在28月龄肥育牛中观察到。如果D-loop对牛肉生产有一定的生理影响，由于D-loop区域不编码任何蛋白质，因此应该存在与D-loop类型协调的D-loop区域以外的区域的突变。我们从每种线粒体类型中选择两种动物，测定线粒体全基因组DNA序列。我们在16 SrRNA中发现了4型特异性突变，可能影响线粒体的蛋白质合成。