APPLICATION OF GENOME ANALYSIS BY AFLP FINGERPRINT TO CATTLE BREEDING

AFLP指纹图谱基因组分析在牛育种中的应用

• 批准号: 13460126
• 负责人: TSUJI Soichi
• 金额: $ 9.79万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460126/
• 关键词: JAPANESE BLACK; MARKER ASSISTED SELECTION; AFLP; BREEDING VALUE; IMPROVEMENT OF REPRODUCTIVE COWS; BREED IDENTIFICATION; SNPs; 牛の品種鑑定; ホルスタイン種; AFLP法; SNP s; 品種鑑定; DNA多型; RDA法

1) BREED IDENTIFICATION; By using DNA markers (SNPs) developed by AFLP fingerprinting, we tried to identify Japanese Black, Holstein and their F1. More than 2000 primer sets were tested between two breeds and found 45 effective breed identifiable AFLP fragments were obtained. By testing 100 animals of each breeds using these 45 AFLP fragments, we could clearly discriminated these animals from each other. This method could prevent or reduce false or miss labeled beef in markets.2) APPLICATION OF AFLP METHOD TO CATTLE BREEDING; By selecting 48 extreme animals from about 5,000 reproductive cows, 25 were the highest breeding value of beef marbling standard (BMS) and 23 were the lowest breeding value of BMS, a kind of association study was done. By AFLP fingerprinting, DNA from those extreme animals were compared about 500 primer sets, which is equivalent to mapping of more than 2,000 markers on a cattle linkage map and finally get 16 effective AFLP fragments which discriminate two extremes, high and low. Quite higher correlation coefficient, r=0.92 were obtained between breeding value of BMS and general genetic value (GGV) estimated using those 16 fragments. This GGV value was applied to additional 121 animals if the GGV is useful or not. Again higher correlation coefficient between BMS breeding value and GGV, r=0.70, was obtained another animals from the herd with an additional higher correlation coefficient, r=0.63, between rib eye muscle area (REA) breeding value and GGV. In generally, higher correlation between BMS and REA is well known, so that this result is reasonable from the data of statistical genetics. This result offers an epoch-making strategy in animal breeding.

1)品种鉴定；利用AFLP指纹图谱建立的DNA标记（snp），对日本黑、荷斯坦及其F1进行了鉴定。在两个品种之间测试了2000多个引物，获得了45个有效的品种可识别的AFLP片段。利用这45个AFLP片段对每个品种的100只动物进行测试，我们可以清楚地区分这些动物。该方法可以防止或减少市场上的假标或误标牛肉。2) aflp方法在奶牛育种中的应用；从约5000头繁殖奶牛中选取48头极端动物，其中25头为牛肉大理石花纹标准（BMS）的最高育种值，23头为BMS的最低育种值，进行相关性研究。利用AFLP指纹图谱技术，对这些极端动物的DNA进行了约500组引物比对，相当于在牛的连锁图谱上定位了2000多个标记，最终得到了16个有效的AFLP片段，分别区分了高、低两个极端。BMS的育种值与利用这16个片段估算的一般遗传值（GGV）有较高的相关系数，r=0.92。如果GGV有用与否，该GGV值将应用于另外121只动物。畜群中BMS育种值与GGV的相关系数也较高，r=0.70，肋眼肌面积（REA）育种值与GGV的相关系数也较高，r=0.63。一般来说，BMS与REA的相关性较高是众所周知的，因此从统计遗传学的数据来看，这一结果是合理的。这一结果为动物育种提供了划时代的策略。

项目成果

Mannen H.: "Mitochondrial DNA reveal that domestic goat (Capra hircus) are genetically affected by two subspecies of bezoar (Capra aegagurus)"Biochem.Genet.. 39. 145-154 (2001)

Mannen H.：“线粒体 DNA 揭示家养山羊 (Capra hircus) 在遗传上受到两种牛黄亚种 (Capra aegagurus) 的影响”Biochem.Genet.. 39. 145-154 (2001)

H. Mannen, M. Morimoto, K. Oyama, F. Mukai, and S. Tsuji: "Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle"J. Anim. Sci.. 81. 68-73 (2003)

H. Mannen、M. Morimoto、K. Oyama、F. Mukai 和 S. Tsuji：“与日本黑牛肉质相关的线粒体 DNA 取代的鉴定”J。

Honda, T. et al.: "Genetic diversity of a closed population of Japanese Black cattle in Hyogo Prefecture"Anim. Sci. J.. 72. 378-385 (2001)

Honda, T. 等人：“兵库县日本黑牛封闭种群的遗传多样性”动画。

Honda, T., Nomura, T., Fukushima, M., and Mukai, F.: "Gene dropping analysis of founder contribution in a closed Japanese Black cattle population"Anim. Sci. J.. 73(2). 105-111 (2002)

Honda, T.、Nomura, T.、Fukushima, M. 和 Mukai, F.：“封闭的日本黑牛种群中创始人贡献的基因下降分析”动画。

Oyama, K. et al.: "Optimization of mating design via a simple genetic algorithm with constrained inbreeing and mating frequency in a long-term selection"Anim. Sci. J.. 72. 279-284 (2001)

Oyama, K. 等人：“通过简单的遗传算法优化交配设计，在长期选择中限制近亲繁殖和交配频率”动画。