Development of New System for Production of Liver Specific Proteins using Cell lines Derived from Human Liver by Radial Flow Type Bioreactor

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As an alternative to liver transplantation surgery, numerous researchers have been working toward the goal of a fully functional artificial liver. In recent years, artificial liver support systems have been advocated as interim treatments for patients awaiting hepatocyte replacement therapy or liver transplantation treatment ; so-called "bridging" treatments. It is recognized that art effective artificial liver system requires : 1) a viable and highly functional hepatocyte cell line, 2) a suitable bioreactor environment and peripheral control systems, and 3) incorporation within an effective extracorporeal circulatory system. Conventional systems have, however, suffered from various drawbacks including incompatibility of cell cultures deriverd from non-human cells, insufficient cell proliferation, rapid deterioration of cellular function due to an impoverished cellular environment, and lack of system scalability. A newly established artificial liver system overcomes many of these problems and demonstrates a long-term capacity to maintain multiple liver-specific functions, such as protein synthesis, enzyme activity and drug metablism, both quantitatively and qualitatively.