DETERMINAT OF RADIAL FLOW BY MR MICROSCOPY IN COAXIAL BIOARTIFICIAL LIVER LOBULE

我们建议使用MRM来研究骨骼的整合 斑点叉尾轮虫颅面发育过程中的神经成分 家蝇(负鼠)。Monodelphis是一种有趣的动物模型 由于口腔骨骼的发育而导致的头面部发育 结构加速(与胎盘哺乳动物相比)，而 神经系统发育迟缓。我们将关注两个时代 --出生日期(相当于胚胎第12天的老鼠)；以及 出生后大约20天。我们会确定是否有 足够的对比度和分辨率(在9特斯拉时) 脊椎动物神经组织和骨骼元素的分化 头。我们希望比较不同的固定强度和时间，在 以寻找这些标本的最佳制备方法。多数 允许评估组织生长的技术要么导致 过度收缩，特别是神经组织的过度收缩(例如，组织学)， 或者破坏骨骼元素(移除大脑并称其重量)。 磁共振显微镜将允许评估大脑发育，而 保存标本以用于研究骨骼的各种技术 差异化。(有关详细信息，请参阅附件 对这项工作的描述和最近关于这方面的出版物的例子 工作)。

We propose to use MRM to investigate the integration of skeletal and neural components during craniofacial development in Monodelphis domestica (opossum). Monodelphis is an interesting animal model for craniofacial development because development of oral skeletal structures are accelerated (compared to placental mammals) while the nervous system is delayed in development. We will focus on two ages -- the day of birth (equivalent to a mouse at embryonic day 12), and approximately 20 days after birth. We will determine whether there is sufficient contrast and resolution (at 9 Tesla) to follow the differentiation of the neural tissues and skeletal elements of the head. We hope to compare various fixation strengths and times, in order to find the optimal preparation of these specimens. Most techniques that allow the assessment of growth of tissues either cause excessive shrinkage, particularly of neural tissue (e.g., histology), or destroy the skeletal elements (removing the brain and weighing it). MR microscopy will allow the assessment of brain growth, while preserving the specimen for various techniques to study skeletal differentiation. (Please see attachment for a more detailed description of this work and examples of recent publications on this work).