DETERMINAT OF RADIAL FLOW BY MR MICROSCOPY IN COAXIAL BIOARTIFICIAL LIVER LOBULE

通过磁共振显微镜测定同轴生物人工肝小叶的径向血流

• 批准号: 6220737
• 负责人: Jeffrey M. Macdonald
• 金额: $ 0.38万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220737
• 关键词: Mammalia; biomedical resource; genetics; growth /development; human tissue; model design /development; nervous system; nuclear magnetic resonance spectroscopy; skeletal system

We propose to use MRM to investigate the integration of skeletal and neural components during craniofacial development in Monodelphis domestica (opossum). Monodelphis is an interesting animal model for craniofacial development because development of oral skeletal structures are accelerated (compared to placental mammals) while the nervous system is delayed in development. We will focus on two ages -- the day of birth (equivalent to a mouse at embryonic day 12), and approximately 20 days after birth. We will determine whether there is sufficient contrast and resolution (at 9 Tesla) to follow the differentiation of the neural tissues and skeletal elements of the head. We hope to compare various fixation strengths and times, in order to find the optimal preparation of these specimens. Most techniques that allow the assessment of growth of tissues either cause excessive shrinkage, particularly of neural tissue (e.g., histology), or destroy the skeletal elements (removing the brain and weighing it). MR microscopy will allow the assessment of brain growth, while preserving the specimen for various techniques to study skeletal differentiation. (Please see attachment for a more detailed description of this work and examples of recent publications on this work).

我们建议使用MRM来研究骨骼整合 Monodelphis颅面发育过程中的神经成分 负鼠（opossum）。 Monodelphis是一种有趣的动物模型， 由于口腔骨骼的发育而引起的颅面发育 结构加速（与胎盘哺乳动物相比）， 神经系统发育迟缓。 我们将重点关注两个年龄段 - 出生日（相当于小鼠胚胎第12天），以及 出生后20天左右。 我们将确定是否有 足够的对比度和分辨率（9特斯拉）， 的神经组织和骨骼元素的分化 头 我们希望比较各种固定强度和时间， 为了找到这些标本的最佳制备方法。 最 允许评估组织生长的技术 过度收缩，特别是神经组织的过度收缩（例如，组织学）， 或破坏骨骼元素（取出大脑并称重）。 MR显微镜将允许评估大脑生长，而 保存标本，以供各种技术研究骨骼 分化 （详细信息请参见附件） 这方面工作的说明和最近出版物的例子 工作）。