Involvement of apoptosis in the detaching process of the epithelial primordium

细胞凋亡参与上皮原基的脱离过程

• 批准号: 10640649
• 负责人: WATANABE Yuichi
• 金额: $ 0.51万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640649/
• 关键词: Apoptosis; Epithelial Rudiment; Rat Fetus; Development; Rathke's Pouch; 器官発生; ラット; 腺性下垂体; 甲状腺

The present study was conducted to investigate to what extent apoptosis is involved in the process of detachment of the adenohypophysial primordium from the oral epithelium. For this purpose, sections including hypophysial rudiment of the 12- to 14-day-old rat fetuses was examined by using an in situ end labelling (TUNEL) method. Massive apoptosis was observed in the oral epithelium when the adenohypophysial primordium closes to form Rathke's pouch. Apoptotic cell death of the oral epithelium was not confined to the closing pouch but it was seen along the midline of the stomodeal roof. In observing closing Rathke's pouch, the apoptotic cells was frequently seen in the oral epithelium itself but it was less frequent in the adoral constricted part. Moreover, the incidence of cell death in the epithelial constriction was variable among individuals. In some fetuses, epithelial cells were found to be incorporated into the hypophysial primordium without degeneration. Such incomplete degenerative process of epithelial component is discussed in relation to the frequent presence of epithelial cysts in the adult rat adenohypophysis.

本研究旨在探讨腺垂体原基从口腔上皮脱落过程中细胞凋亡的程度。为此目的，通过使用原位末端标记（TUNEL）方法检查了12至14日龄大鼠胎仔的包括垂体原基在内的切片。当腺垂体原基关闭形成Rathke囊时，口腔上皮细胞大量凋亡。口腔上皮细胞的凋亡并不局限于封闭袋，但它被视为沿着中线的造口处理屋顶。在Rathke囊闭合的观察中，凋亡细胞多见于口腔上皮本身，而在口侧收缩部较少见。此外，上皮收缩中细胞死亡的发生率在个体之间是可变的。在一些胎儿中，发现上皮细胞被并入垂体原基而不变性。这种不完全的上皮成分的退化过程中讨论的关系，在成年大鼠腺垂体上皮囊肿的频繁存在。

项目成果

Matsumoto: "Differentiating ability of the parts intermedia in hypothelectomized frog tadpoles"General and Comparative Enclocrinology. 117巻(予定). (2000)

松本：“假肢青蛙蝌蚪的中间部分的分化能力”，普通和比较内分泌学第 117 卷（计划）。

Hosoya: "Immunocytochemical studies on the cellular origin of ectopic striated muscle fibers in monolayer cultures of rat"Zoological Science. 15巻2号. 247-253 (1998)

Hosoya：“大鼠单层培养物中异位横纹肌纤维细胞起源的免疫细胞化学研究”《动物学科学》，第 15 卷，第 2 期，247-253（1998 年）。

Sato: "Corticosteroids stimulate the differentiation of growth hormone cells but suppress that of prolactin cells in the fetal rt pituitary"Archives Histodogy and Cytology. 61. 75-81 (1998)

佐藤：“皮质类固醇刺激生长激素细胞的分化，但抑制胎儿 rt 垂体中催乳素细胞的分化”档案组织学和细胞学。

Sato: "Corticosteroids stimulate the differentiation of growth hormone cells but suppress that of prolactin cells in the fetal rat pituitary"Archives Histology and Cytology. 61巻1号. 75-81 (1998)

Sato：“皮质类固醇刺激胎鼠垂体中生长激素细胞的分化，但抑制催乳素细胞的分化”，档案组织学和细胞学，第 61 卷，第 1. 75-81 期（1998 年）。

Hosoya: "Immunocytochemical studies on the cellular origin of ectopic striated muscle fibers in monolayer cultures of rat"Zoological Science. 15. 247-253 (1998)

Hosoya：“大鼠单层培养物中异位横纹肌纤维细胞起源的免疫细胞化学研究”《动物学科学》。