Three-dimensional analysis for neural network of photosensory pineal organ

松果体光感神经网络的三维分析

• 批准号: 10640663
• 负责人: TAMOTSU Satoshi
• 金额: $ 2.18万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640663/
• 关键词: Pineal organ; Photosesory; Three-dimensional structure; Photoneuroendocrine; Lamprey; Photoreceptor cell; 神経節細胞; 蛍光多重標識; 光感覚器官; 神経回路網

In the pineal organ of river lamprey, Lampetra japonica, there are two types pineal photoreccptors, typical and modified, which are regarded as the pure photoreceptor cell like visual cells in retina and the photoneuroendocrine cell secreting the melatonin, respectively. We investigate for the structure and the subtype of pineal photoreceptors and ganglion cells and their neural network three-dimensionally by use of a fluorescent labeling technique and a confocal laser-scanning microscope. The results are summarized below.1. The photoneuroendocrine cell, which shows serotonin immunoreactivity, possesses a basal process, and it appears that the process does not branch out. These processes contact each other at the basal region of the end vesicle, .2. Most of serotonin-immunoreactive cells showed iodopsin immunoreactivity, but not all. The results suggest that the iodopsin (red-cone visual pigment)-immunoreactive cells are predominant in the end-vesicle of lamprey pineal organ as neuroendocrine cells.3. Some visinin (cone-specific Ca2+binding protein)-immunoreactive cells show no iodopsin immunoreactivity. Many visinin-immunoreactive cells showed rhodopsin immunoreactivity. The results suggest that another cone-type photoreceptor exits in the pineal complex besides iodopsin immunoreactive cells.4. Globular and large ganglion cells were mainly located at peripheral region of the end vesicle. The ganglion cells connecting with the visinin-immunoreactive cells were globular in shape and large in size.

在七鳃鳗的松果体器官中，存在典型型和变异型两种类型的松果体感光细胞，分别被视为视网膜中的纯感光细胞（如视觉细胞）和分泌褪黑激素的感光神经内分泌细胞。我们利用荧光标记技术和共焦激光扫描显微镜，对松果体感光细胞和神经节细胞的结构和亚型及其神经网络进行三维研究。研究结果如下： 1．显示血清素免疫反应性的光神经内分泌细胞具有一个基础过程，并且该过程似乎不会分支。这些过程在末端囊泡的基底区域相互接触，0.2。大多数血清素免疫反应性细胞显示出碘紫红质免疫反应性，但不是全部。结果表明，七鳃鳗松果体终泡中以碘紫红质（红锥视色素）免疫反应细胞为主，为神经内分泌细胞。3．一些维西宁（锥体特异性 Ca2+ 结合蛋白）免疫反应性细胞不显示碘紫红质免疫反应性。许多维西宁免疫反应性细胞表现出视紫红质免疫反应性。结果表明，松果体复合体中除碘紫红质免疫反应细胞外，还存在另一种视锥型光感受器。 4．球状神经节细胞和大神经节细胞主要位于终泡的周边区域。与visinin免疫反应细胞连接的神经节细胞呈球状且尺寸较大。

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