Release characteristics of antibacterial substances included in cyclodextrin

具有功能性表面蛋白的细胞，如纤维素结合蛋白和葡萄糖淀粉酶，在固定化酶和食品工业中是有用的。Geotrichum sp.细胞M111具有加速纤维素纤维聚集的能力。研究了真空干燥和喷雾干燥M111细胞的生长特性及纤维素粉聚集能力的变化。得到了以下结果：1)考察了温度、pH、搅拌速度和曝气速度对M11细胞生长特性的影响。搅拌速度对M11细胞的生长速率有显著影响，但与空气流速无关。生长的最适温度为27℃，pH为5℃。2)通过在玻璃微管中测定纤维素粉的聚集高度，表征了纤维素粉与酵母样细胞M111的聚集能力。聚集体的最适pH值为4 ~ 5℃，温度为50℃以下。M111电池的加热变性与一级变性动力学密切相关。变性活化能为213kJ/mol。SDS或sts等表面活性剂可抑制细胞聚集。3)80℃真空干燥M111细胞。真空干燥细胞的纤维素聚集能力没有变化，保持与原始细胞相近的水平。4)采用喷雾干燥法对M111细胞进行干燥。进风口温度为150 oC，出风口温度为79oC。在这种情况下，喷雾干燥细胞的纤维素聚集能力下降到原始能力的80%。这表明纤维素结合能力的下降是由于在雾化过程中剪切应力的作用。

The cell with a functional surface protein such as cellulose-binding protein and glucoamylase is useful to the immobilized enzyme and food industry. Geotrichum sp. cell, M111 has an ability to accelerate the aggregation of cellulose fiber. We investigated the growth characteristics of M111 cells and the change of the aggregation ability of cellulose powder of vacuum and spray dried M111 cells. The following results were obtained.1)The effects of temperature, pH, agitation speed, and the aeration velocity on the growth characteristics of M11 cells were investigated. The growth rate of M11 cells was markedly affected by the agitation speed, but was independent of the flow rate of air. The optimum temperature and pH for growth were 27 oC and 5, respectively.2)The aggregation ability of cellulose powder with a yeast-like cell of M111 was characterized by the measurement of the aggregation-height in a glass micro-tube. The optimum pH and temperature of the aggregation were pH 4-5 and below 50 degree. The denaturation of M111 cell by heating could be well correlated with a first order denaturation kinetics. The activation energy of denaturation was 213kJ/mol. The aggregation was inhibited with surfactants such as SDS or STS.3)M111 cells were dried at 80 oC under vacuum. The cellulose aggregation ability of the vacuum dried cells did not change and maintained the similar level to the original cells.4)Spray drying method was applied to make a dried cell of M111. The inlet and the outlet temperature of air were 150 oC and 79oC, respectively. In this case, the cellulose aggregation ability of the spray-dried cells was decreased to 80% of the original ability. This implied that the decrease of the cellulose binding ability resulted from the shear stress at atomization.