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Release characteristics of antibacterial substances included in cyclodextrin

环糊精中抗菌物质的释放特性

基本信息

批准号：
10650787
负责人：
FURUTA Takeshi
金额：
$ 1.86万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650787/
关键词：
surface protein Getrichum sp., M111 cells spray drying cellulose-binding ability

项目摘要

The cell with a functional surface protein such as cellulose-binding protein and glucoamylase is useful to the immobilized enzyme and food industry. Geotrichum sp. cell, M111 has an ability to accelerate the aggregation of cellulose fiber. We investigated the growth characteristics of M111 cells and the change of the aggregation ability of cellulose powder of vacuum and spray dried M111 cells. The following results were obtained.1)The effects of temperature, pH, agitation speed, and the aeration velocity on the growth characteristics of M11 cells were investigated. The growth rate of M11 cells was markedly affected by the agitation speed, but was independent of the flow rate of air. The optimum temperature and pH for growth were 27 oC and 5, respectively.2)The aggregation ability of cellulose powder with a yeast-like cell of M111 was characterized by the measurement of the aggregation-height in a glass micro-tube. The optimum pH and temperature of the aggregation were pH 4-5 and below 50 degree. The denaturation of M111 cell by heating could be well correlated with a first order denaturation kinetics. The activation energy of denaturation was 213kJ/mol. The aggregation was inhibited with surfactants such as SDS or STS.3)M111 cells were dried at 80 oC under vacuum. The cellulose aggregation ability of the vacuum dried cells did not change and maintained the similar level to the original cells.4)Spray drying method was applied to make a dried cell of M111. The inlet and the outlet temperature of air were 150 oC and 79oC, respectively. In this case, the cellulose aggregation ability of the spray-dried cells was decreased to 80% of the original ability. This implied that the decrease of the cellulose binding ability resulted from the shear stress at atomization.

具有纤维素结合蛋白和葡糖淀粉酶等功能性表面蛋白的细胞可用于固定化酶和食品工业。Geotrichum sp.细胞M111具有促进纤维素纤维聚集的能力。研究了M111细胞的生长特性以及真空干燥和喷雾干燥后细胞纤维素粉末聚集能力的变化。主要研究结果如下：1）研究了温度、pH、搅拌速度和通气速度对M11细胞生长特性的影响。M11细胞的生长速率受搅拌速度的影响显著，但与空气流速无关。最适生长温度为27 ℃，最适生长pH为5。2）通过测定纤维素粉末在玻璃微管中的聚集高度，研究了纤维素粉末与M111酵母样细胞的聚集能力。聚结的最适pH值为4-5，最适温度为50 ℃以下。M111细胞的热变性符合一级动力学。变性反应的活化能为213 kJ/mol。用表面活性剂如SDS或STS抑制聚集。3）将M111细胞在80 ℃真空下干燥。真空干燥细胞的纤维素聚集能力没有改变，并保持与原始细胞相似的水平。4）应用喷雾干燥法制备M111的干燥细胞。空气的入口和出口温度分别为150 ℃和79 ℃。在这种情况下，喷雾干燥的细胞的纤维素聚集能力降低到原始能力的80%。这表明，雾化时的剪切应力导致纤维素结合能力的降低。