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Fundamental Study of Surface Plasmon Resonance and Analysis of Biomolecular based on the Antigen-antibody Reactions

基于抗原抗体反应的表面等离子共振和生物分子分析的基础研究

基本信息

批准号：
10650800
负责人：
KAMATA Satsuo
金额：
$ 1.92万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650800/
关键词：
Surface plasmon resonance sensor Thin gold film ABO blood type Reaction of antigen-antibody coupling Saliva Bloodstain Tumor antigen α-fetoprotein (AFP)Pesticide 表面プラズモン共鳴金属薄膜 ABO血液型簡易測定尿塩化ベンゼトニウム

项目摘要

The surface plasmon resonance (SPR) method is a powerful tool for determining the optical properties of thin dielectric layers. In the SPR method, the angular dependence of the reflectivity from a dielectric surface (gold film) is studied in the region to total internal reflections. Surface plasmon is a surface charge-density wave at a metal surfaces attached sample. The evanesent, wave is produced to the interface between the prism and slide glass coated with thin gold film. At critical angle, surface plasmon is exited by resonance with evanesent, wave and then the reflectivity is decrease sensitively. The resonance angle is very sensitive to variations in the refractive index of the medium just outside the metal thin film. So we measured the refractive index of the biomolecules to be analyze by the SPR method. In this project, we investigated first the effect of SPR response for the thickness change of metal film and the flatness which was observed by AFM. Under the appropriate condition, we studied the analysis of some biomolecules as follows.l) ABO blood type of bloodstain and saliva were measured by the reaction of monoclonal antibody with antigen sample on the thin gold film rapidly and sensitively. 2) The tumor antigen, α-fetoprotein (AFP), in serum was analyzed sensitively and rapidly in the region of 1 IU/ml to 20 IU/ml. 3) The gold-coated unclad optical fiber responded rapidly to the ethanol and ethylene glycol by the SPR mechanism.

表面等离子体共振（SPR）方法是测量薄介质层光学特性的有力工具。在SPR方法中，从一个介质表面（金膜）的反射率的角度依赖性进行了研究，在该区域的全内反射。表面等离子体激元是附着在金属表面的样品的表面电荷密度波。在棱镜与镀有薄金膜的载玻片之间的界面上产生倏逝波。在临界角处，表面等离子体激元与倏逝波共振激发，反射率急剧下降。谐振角对金属薄膜外介质折射率的变化非常敏感。因此，我们用SPR方法测量了待分析生物分子的折射率。在本计画中，我们首先研究了表面电浆共振效应对金属薄膜厚度变化及原子力显微镜所观察到的平坦度的影响。在适当的条件下，我们对一些生物分子的分析进行了如下研究：1）用单克隆抗体与抗原样品在金膜上反应，快速、灵敏地测定了血痕和唾液中的ABO血型。2)在1 ~ 20 IU/ml范围内，对血清中肿瘤抗原甲胎蛋白（AFP）进行了灵敏、快速的分析。3)通过SPR机制，镀金无包层光纤对乙醇和乙二醇有快速响应。