Study on the conservation of genetic diversity in Calanthe triplicata

三瓣虾兰遗传多样性保护研究

基本信息

批准号：
10660039
负责人：
MIZUTANI Takayuki
金额：
$ 1.86万
依托单位：
KYUSHU TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Ten of thirteen isozymes used for the analysis in Calanthe triplicata, DIA, FDH, GDH, GOT, MDH, ME, 6-PDH, PGI, PGM, and SADH, showed the stable zymogram patterns. Of these, seven enzymes, GOT, IDH, MDH, ME, PGI, 6-PDH and SADH, showed intraspecific polymorphisms in C.triplicata. Especially, GOT, MDH and 6-PDH were found showing highly comparing with other terrestrial orchids grow wild in Iriomote Island might contribute the wide distribution of this species. All individual plants of Calanthe triplicata included in three populations were analyzed by seven isozymes systems. In all the three populations, the big diversities of the isozymes were observed. Most of plants investigated showed the variation and the high heterozygousity in the population of C.triplicata. Seventeen of eighteen individuals in the population B can be identified using only four or five isozyme systems. The isozyme variation observed in the population B showed that the leading propagation strategy of the C.triplicata is seedling. Only two plants could not be distinguished from each other by the seven isozyme systems. They located very closely in the population, suggesting that the two plants elonged to the same colony or one of them was an offspring or a pod mate of the other. The considered to be maintained by a seed propagation, especially outbreeding. The species was found to have a big genetic diversity even in a wild population, suggesting that Calanthe triplicata is an allogamous species propagating itself by seedling.

在Calanthe Triplicata，DIA，FDH，GDH，GOT，MDH，ME，6-PDH，PGI，PGM和SADH中用于分析的13个同工酶中的十三个同工酶显示出稳定的Zymogram图模式。在这些酶中，有7种酶，IDH，MDH，ME，PGI，6-PDH和SADH在c.triplicata中显示出种内多态性。尤其是，发现与其他陆地兰花在Iriomote Island中野生生长的GOT，MDH和6-PDH可能贡献了该物种的广泛分布。 Calan的所有单个植物通过七个同工酶系统分析了三个种群中的三分之一。在所有三个人群中，都观察到同工酶的巨大多样性。所研究的大多数植物都表明c. triplicata种群中的差异和高杂合性。人口B中的18个人中有17个只能使用四或五个同工酶系统识别。人群B中观察到的同工酶变异表明，c.triplicata的主要传播策略是幼苗。七个同工酶系统只能彼此区分两种植物。他们非常紧密地位于人口中，这表明两种植物延伸到同一个菌落或一个是后代或另一个豆荚伴侣。被认为是通过种子繁殖而维持的，尤其是育种。发现该物种即使在野生人群中也具有巨大的遗传多样性，这表明Calanthe Triplicata是一种通过幼苗传播的同属物种。