Development of Biocatalytic Functional Column Electrolytic Method for Application to High-Performance Biotransformation and Absolute and Sensitive Analysis.

将注意力集中在从烟酸（NA）中将6-羟基烟酸（6 HNA）转化为假单胞菌荧光TN-5中的生物转化系统中，并将电子从细胞转移到柱的电子已经被用作异基因受体的非甾体。The hydoxylation is remarkably accelerated using such exogenous electron transfer mediator， in place of the native final electron acceptor OイイD22イエD2。在添加中，6 HNA的成功反应可以完全被使用的exogenous electron transfer mediator所完全抑制。根据结果，一个电化学加速和100%的生物转换系统已经在我们的列电化学系统上实现了现实化，其中假单胞菌fluorescens TN-5细胞在列电化学上实现了非移动性。6 HNA的完整生物转化是第一种情况。这个方法可以应用于6 HNA作为农药的一种重要的启动材料的生产。在添加中，这个方法可以成功地应用于对单个细胞中的NA脱氢酶数量的评估，在该柱上的酶免疫力被减少时，然后在该柱上的酶被电化学重新氧化的地方，根据一个类似原则，NA是在荧光假单胞菌TN-5细胞-不移动的柱电中被氧化。冷计量分析允许NA降低到0.2 pmol的高度敏感和绝对确定性。

Focusing our attention to a biotransformation system from nicotinic acid (NA) into 6-hydroxynicotinic acid (6HNA) by Pseudomonas fluorescens TN-5, the electron transfer from the cell to column electrodes has been realized using ferricyanide as an exogenous electron acceptor. The hydoxylation is remarkably accelerated using such exogenous electron transfer mediator, in place of the native final electron acceptor OィイD22ィエD2. In addition, the succeeding reaction of 6HNA can be completely suppressed by the use of the exogenous electron transfer mediator.Based on the results, an electrochemically accelerated and 100% biotransformation system has been realized on our column electrolytic system, where Pseudomonas fluorescens TN-5 cells were immobilized on a column electrode. Such complete biotransformation of NA into 6HNA is the first case. This method would be applied to the production of 6HNA as an important starting material of pesticides.In addition, this method can be successfully applied to the evaluation of the number of NA dehydrogenase in a single cell, where the enzyme immobilized on the column is reduced with NA and after then the reduced enzyme is electrochemically re-oxidized.Based on a similar principle, NA is bioelectrochemically oxidized at Pseudomonas fluorescens TN-5 cell-immobilized column electrode. The coulometric analysis has allowed highly sensitive and absolute determination of NA down to 0.2 pmol.