CHARACTERIZATION OF CELLULOSE-BINDING ABILITY OF GEOTRICHUM SP., M111 CELL AND APPLICATION OF DEHYDRATION FOR DISTILLED

用微管法研究了土曲（Geotrichum sp., M111）细胞的纤维素结合能力和聚集能力。测定纤维素粉与M111细胞的聚合高度时，最适pH值为4 ~ 5℃，温度为50℃以下。M111细胞与纤维素纤维的结合常数为0.3%，在20mM柠檬酸缓冲液中，pH5.0, 30℃。表面活性剂如十二烷基硫酸钠可抑制其聚集。M111细胞与纤维素纤维的结合能力随着driselase和pronase e的处理而消失，这表明这种结合能力可能与细胞表面蛋白有关。M111细胞的加入加快了甘薯烧酒蒸馏废液的脱水速率。用线性粘弹性模型对脱水过程进行分析，认为加速效应可能是由于纤维素纤维之间的空间随着细胞的加入而增加。

Cellulose-binding ability of Geotrichum sp., M111 cell was investigated as the aggregation ability with the microtube method. The optimum pH and temperature were pH 4-5 and below 50℃ with measurement of the aggregation height for a mixture of cellulose powder and M111 cell. The binding constant of M111 cell to cellulose fiber was obtained 0.3% in 20mM citrate buffer, pH5.0 at 30℃. The aggregation was inhibited with surfactants such as sodium dodecylsulfate. The binding ability of M111 cell to cellulose fiber disappeared with the treatment of driselase and pronase E. This suggests the binding ability might be due to the cell surface protein. The dehydration rate of distilled waste of sweet potato shochu was accelerated with the addition of M111 cell. The analysis of dehydration with the linear viscoelastic model suggests the acceleration effect might be due to the space increment between cellulose fiber with the cell addition.