Relationship wound healing and molecular weight/deacetylation of poly amino sugar.

伤口愈合与聚氨基糖的分子量/脱乙酰化的关系。

基本信息

批准号：
10660301
负责人：
YOSHIHARU Okamoto
金额：
$ 2.24万
依托单位：
TOTTORI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660301/
关键词：
AMINO SUGAR CHITIN CHITOSAN FIBROBLAST VASCULAR ENDOTHELIUM COLLAGEN MIGRATION 創傷治癒促進肉芽形成引っ張り強度

项目摘要

1. Effects of molecular weight and deacetylation of chitin/chitosan on incisional wound in rats : Regardless of molecular weight and deacetylation of chitin/chitosan, tensile strength of incisional wound increased significantly in the presence of chitin and chitosan at 4 days post-wounding. Microscopically, inflammatory reaction at the wound site was observed in the higher concentration groups of chitin and chitosan. The activity of prolyl hydroxylase was high in the higher concentration groups of chitin and chitosan.2. Effects of chitin/chitosan and their oligomers and monomers on fibroblast and vascular endothelium : In direct migratory assay using the blind well chamber method, migratory activity of fibroblasts (3T6) was seen to be reduced by chitin, chitosan and the chitosan monomer (GlcN). Migratory activity of vascular endothelial cells (HUVECs) was enhanced by chitin, chitosan and the chitin monomer (GlcNAc), and was reduced by chitosan oligomers and GlcN.Supernatant of 3T6 preincubated with chitin or chitosan reduced migratory activity of 3T6 cells. Supernatant of HUVECs preincubated with chitosan also reduced migratory activity of HUVECs, but supernatant preincubated with chitin had no effect on them. In a proliferation (MTT reduction) assay, none of the samples affected proliferation of either type of cell.3. Effects of chitin/chitosan on prolyl hydroxylase activity : The acitivity of prolyl hydroxylase activity in each group was found to be low until 4 days post-implantation (Day4). However, in the 10 mg chitin-NWF group, its activity increased rapidly at Day 7 and remained at a plateau until Day 14. In other groups, their acitivities increaed linearly until Day 14.

1。壳蛋白/壳聚糖分子量和脱乙酰基化对大鼠切口伤的影响：无论分子量的体重如何，甲壳质/壳聚糖的脱乙酰基化，切口伤口的拉伸强度在后4天的壳质和壳聚糖的存在下显着增加。在显微镜下，在几丁质和壳聚糖的较高浓度组中观察到伤口部位的炎症反应。在几丁质和壳聚糖浓度较高的基团中，羟基羟化酶的活性很高。2。几壳/壳聚糖及其低聚物以及单体对成纤维细胞和血管内皮的影响：在使用盲孔室法直接迁移测定中，成纤维细胞（3T6）的迁移活性被壳聚糖，壳聚糖，壳聚糖和壳聚糖单体（GLCN）降低。血管内皮细胞（HUVEC）的迁移活性通过几丁质，壳聚糖和几壳蛋白单体（GLCNAC）增强，并通过3T6的3T6壳糖浆低聚物和glcn降低，并用壳蛋白或壳聚糖减少3T6细胞的迁移活性。与壳聚糖预孵育的HUVEC的上清液也降低了HUVEC的迁徙活性，但是与甲壳质预孵育的上清液对它们没有影响。在增殖（MTT还原）测定中，没有任何样品影响两种细胞的增殖。3。几丁质/壳聚糖对羟基羟化酶活性的影响：发现每组中丙酰羟化酶活性的耐药性较低，直到植入后4天（第4天）。但是，在10 mg的几丁质-NWF组中，其活性在第7天迅速增加，并一直处于高原直到第14天。在其他组中，其敏捷性逐渐递增至第14天。