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Utilization of biomass resources-environmental clean up with plant proteins and microorganisms-

生物质资源利用-植物蛋白和微生物净化环境-

基本信息

批准号：
10660314
负责人：
KUBO Motoki
金额：
$ 2.24万
依托单位：
Ritsumeikan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660314/
关键词：
Biomass Activition of rost hair Plant growth stimulation factor Plant protein Bacillus Streptomyces ぺプチド土壌微生物ペプチド Bacillus circulans Streptomyces sp.

项目摘要

<<Plant growth stimulation with soybean proteins>>・ Microorganisms having potential for soybean degradation within 48 h were isolated.・ These bacteria were identified as Bacillus circulans, B.stearothermophilus, Streptomyces sp., respectively.・ The degradation products (DSP) have plant growth stimulation factors, which were almost equal level as chemically synthesized fertilizer.・ The DSP production process with 2001 jar fermentor was established.<<Analysis of plant growth stimulation factors>>The plant growth stimulation factors from DSP were analyzed. First of all, soybean proteins and the degradation products with HCl were analyzed for plant growth. However, these products have no activity. The plant growth activity was also not detected with soybean proteins degraded products using several proteases. These results indicate that plant growth stimulation factors were originally from soybean degradation products with HA12, HA19, and MF20. The plant growth stimulation factor from HA12 was isolated as single peak with reverse phase chromatography.<<Quantification of microorganisms using nucleic acids from soil>>Time DSP from this research also activate microorganisms in soils. Establishment of quantification of microorganisms using nucleic acids from soils was tried. Efficient nucleic acids isolation method from soils was investigated. As a result, the relationship between quantification of nucleic acids and microorganisms was determined, and a new quantification of soil microorganisms method was established with nucleic acids from soils.

<<Plant growth stimulation with soybean proteins>>·分离出在48 h内具有大豆降解潜力的微生物。·这些细菌被鉴定为环状芽孢杆菌、嗜热脂肪B、链霉菌属、分别地。降解产物（DSP）具有与化学合成肥料相当的植物生长刺激因子。建立了2001罐发酵生产DSP的工艺。<<Analysis of plant growth stimulation factors>>分析了DSP对植物生长的促进作用。首先，分析大豆蛋白和HCl降解产物对植物生长的影响。但是，这些产品没有活性。使用几种蛋白酶的大豆蛋白降解产物也没有检测到植物生长活性。这些结果表明，植物生长刺激因子最初来自大豆降解产物HA 12、HA 19和MF 20。用反相色谱法从HA_（12）中分离得到一种单一峰的植物生长刺激因子。<<Quantification of microorganisms using nucleic acids from soil>>该研究的Time DSP还可以激活土壤中的微生物。尝试建立土壤核酸定量微生物的方法。研究了从土壤中提取核酸的有效方法。确定了核酸定量与微生物定量之间的关系，建立了一种利用土壤核酸定量土壤微生物的方法。