Utilization of biomass resources-environmental clean up with plant proteins and microorganisms-

&lt;<Plant growth stimulation with soybean proteins>&gt;&gt;微生物在48小时内对大豆退化的潜力，而微生物在48小时内被隔离。这些细菌被鉴定为Bacillus circulans， B.stearothermophilus，链霉菌属sp.，尊敬的.....。退化产品（DSP）有植物生长刺激因素，其水平几乎是化学合成的发酵剂。2001年罐子充电器的DSP生产过程已被证实。&lt;<Analysis of plant growth stimulation factors>&gt;植物生长刺激因素从DSP中分析过。首先是所有植物生长的大豆蛋白和降解产品，用HCl进行了分析。好吧，这些产品没有活动。植物生长活动也没有被检测到使用多种蛋白质降解的大豆蛋白质产品。这些结果表明，植物生长刺激因素最初来自大豆退化产品与HA12、HA19和MF20。来自HA12的植物生长刺激因子与反向相位色谱法隔离，达到单峰值。&lt;<Quantification of microorganisms using nucleic acids from soil>&gt;来自本研究的时间DSP也激活土壤中的微生物。对微生物使用核酸从土壤中试验过的核酸进行量化的建立。有效的核酸隔离方法是从土壤中提取的。作为一个结果，核酸和微生物的定量之间的关系已经确定，土壤微生物方法的新定量已经通过土壤中的核酸得到证实。

<<Plant growth stimulation with soybean proteins>>・ Microorganisms having potential for soybean degradation within 48 h were isolated.・ These bacteria were identified as Bacillus circulans, B.stearothermophilus, Streptomyces sp., respectively.・ The degradation products (DSP) have plant growth stimulation factors, which were almost equal level as chemically synthesized fertilizer.・ The DSP production process with 2001 jar fermentor was established.<<Analysis of plant growth stimulation factors>>The plant growth stimulation factors from DSP were analyzed. First of all, soybean proteins and the degradation products with HCl were analyzed for plant growth. However, these products have no activity. The plant growth activity was also not detected with soybean proteins degraded products using several proteases. These results indicate that plant growth stimulation factors were originally from soybean degradation products with HA12, HA19, and MF20. The plant growth stimulation factor from HA12 was isolated as single peak with reverse phase chromatography.<<Quantification of microorganisms using nucleic acids from soil>>Time DSP from this research also activate microorganisms in soils. Establishment of quantification of microorganisms using nucleic acids from soils was tried. Efficient nucleic acids isolation method from soils was investigated. As a result, the relationship between quantification of nucleic acids and microorganisms was determined, and a new quantification of soil microorganisms method was established with nucleic acids from soils.