Physical Interaction between the lnositol 1, 4, 5-Trisphosphate Receptor and Ryanodine Receptor in Rat Cerebellum

大鼠小脑肌醇 1,4,5-三磷酸受体与 Ryanodine 受体之间的物理相互作用

• 批准号: 10670088
• 负责人: ONOUE Hitoshi
• 金额: $ 2.24万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670088/
• 关键词: Ca^<2+>; IP_3R; RYR; subunit; immunoprecipitation; tetramer; hetero; subtype; Ca^<2+>放出チャネル; IP_3R; RYR

Many cell types, including cerebellar Purkinje cell, co-express the inositol 1, 4, 5-trisphosphate receptor and ryanodine receptor, the two classes of the intracellular Ca^<2+> release channel. Functional co-operation between these two intracellular Ca^<2+> release channels seems to exist in such cells. Spatial proximity of the two channels would be important for them to cooperate. Here we report that a physical interaction between subunits of the inositol 1, 4, 5-trisphosphate receptor and ryanodine receptor channel exists. Type 1 inositol 1, 4, 5-trisphosphate receptor subunit co-precipitated when type 2 ryanodine receptor subunit was immunoprecipitated from rat cerebellum membrane fraction by and, vice versa, type 2 ryanodine receptor subunit co-immunoprecipitated with type 1 inositol 1, 4, 5-trisphosphate receptor subunit. When protein complexes in the membrane fraction were dissociated into individual subunits prior to immunoprecipitation procedure, the co-immunoprecipitation of the two Ca^<2+> release channel subunits was abolished despite immunoprecipitating antibodies (specific for type 1 inositol 1, 4, 5-trisphosphate receptor or ryanodine receptor) still immunoprecipitated their respective antigen. Our results suggest a physical association of the inositol 1, 4, 5-trisphosphate receptor and ryanodine receptor, which would ensure the functional co-operation of the two intracellular Ca^<2+> release channels.

许多细胞类型，包括小脑浦肯野细胞，共同表达肌醇1,4,5-三磷酸受体和兰尼碱受体，这两类细胞内Ca 2+ 释放通道。这两个细胞内Ca 2+ 释放通道之间的功能协作似乎存在于此类细胞中。两个渠道的空间接近性对于他们的合作非常重要。在这里，我们报道肌醇 1,4,5-三磷酸受体亚基和兰尼碱受体通道之间存在物理相互作用。当 2 型兰尼碱受体亚基从大鼠小脑膜级分中免疫沉淀时，1 型肌醇 1,4,5-三磷酸受体亚基共沉淀，反之亦然，2 型兰尼碱受体亚基与 1 型肌醇 1,4,5-三磷酸受体亚基共免疫沉淀。当膜级分中的蛋白质复合物在免疫沉淀过程之前解离成单独的亚基时，两个Ca 2+ 释放通道亚基的共免疫沉淀被消除，尽管免疫沉淀抗体（对1型肌醇1,4,5-三磷酸受体或兰尼碱受体具有特异性）仍然免疫沉淀其各自的抗原。我们的结果表明肌醇1,4,5-三磷酸受体和兰尼碱受体的物理关联，这将确保两个细胞内Ca 2+ 释放通道的功能协作。

项目成果

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