Relationship between phosphodiesterase I activity and metastatic ability of cancer cells

磷酸二酯酶I活性与癌细胞转移能力的关系

• 批准号: 10670142
• 负责人: SANO Kimihiko
• 金额: $ 1.66万
• 依托单位: Kobe University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670142/
• 关键词: nucleotide pyrophosphatase; phosphodiesterase I; autotaxin; PC-1; bone metabolism; metastasis; ecto-enzyme; transcriptional regulation; transcriptional regulation; ホスホジエステラーゼI; グリオーマ; エクトエンザイム; PDNP

Nucleotide pyrophosphatase/phosphodiesterase I is an ecto-enzyme catalyzing a hydrolysis of extracellular ATP, and consists of a three homologous molecules, namely, PC-1, PD-Iα/autotaxin, and PD-Iβ/gp130ィイD1RB14-6ィエD1/B10. We have proposed the nomenclature for these molecules, E-NPP1, -2, and -3, respectively. We have previously reported that E-NPP2 is expressed in neuroblastoma tissues, and its expression level is related to the clinical stage of the patients. We have now shown that E-NPP3 is expressed in glioma cells. We also analyzed the promoter activity of the E-NPP3 gene and determined the region required for cell-type specific transcription of this gene. The mouse carrying a mutation in the E-NPP1 gene shows a phenotype resembling human disease, the ossification of the longitudinal ligament of the spine, indicating that E-NPP1 is involved in the regulation of bone formation. We showed that both E-NPP1 and -3 are expressed in chondrocytes at different subcellular localization. E-NPP1 is mostly localized in the plasma membrane whereas E-NPP3 is localized in the Golji body as well as plasma membrane. Both molecules increased a production of adenosine and pyrophosphate in chondrocytes.

核苷酸焦磷酸酶/磷酸二酯酶I是一种催化胞外三磷酸腺苷水解酶的胞外酶，由三个同源分子组成，即PC-1、PD-Iα/Autoaxin和PD-Iβ/gp130ィイD1RB14-6ィエD1/B10。我们提出了这些分子的命名，分别为E-NPP1、-2和-3。我们已经报道了E-NPP2在神经母细胞瘤组织中的表达，其表达水平与患者的临床分期有关。我们现在已经证明E-NPP3在胶质瘤细胞中表达。我们还分析了E-NPP3基因的启动子活性，并确定了该基因的细胞型特异性转录所需的区域。携带E-NPP1基因突变的小鼠表现出类似人类疾病的表型，即脊柱纵向韧带的骨化，表明E-NPP1参与了骨形成的调节。我们发现E-NPP1和-3在不同亚细胞定位的软骨细胞中都有表达。E-NPP1主要定位于质膜，而E-NPP3除定位于质膜外，还定位于高尔基体。这两种分子都增加了软骨细胞中腺苷和焦磷酸的产量。

项目成果

Andoh, K. et al.: "Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells"Biochem. Biophys. Acta. 1446. 213-224 (1999)

Andoh, K. 等人：“神经胶质细胞中表达的外切磷酸二酯酶 I 基因 (PDNP3) 的基因组结构和启动子分析”Biochem。

Andoh,K., Piao,J.-H., Terashima,K., Nakamura,H., and Sano,K.: "Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3 expressed in glial cells"Biochim. Biophys. Acta. 1446. 213-224 (1999)

Andoh,K.、Piao,J.-H.、Terashima,K.、Nakamura,H. 和 Sano,K.：“外切磷酸二酯酶 I 基因（在神经胶质细胞中表达的 PDNP3）的基因组结构和启动子分析”Biochim

Hasegawa D., Sano K., Kosaka Y., Hayakawa A., and Nakamura H.: "A case of hemophagocytic lymphohistiocytosis with prolonged remission after syngeneic bone marrow transplantation"Bone Marrow Transplant. 24(4). 425-428 (1999)

Hasekawa D.、Sano K.、Kosaka Y.、Hayakawa A. 和 Nakamura H.：“同基因骨髓移植后长期缓解的噬血细胞性淋巴组织细胞增多症病例”骨髓移植。

Hasegawa,D et al.: "A case of hemoghagocytic by mphohistio cytosis with prolouged remission after syngeneic bone marrow transplantation"Bone Marrow Transplantation. 24. 425-428 (1999)

Hasekawa,D 等人：“同基因骨髓移植后因淋巴组织细胞增多而导致血细胞增多且缓解期延长的病例”骨髓移植。

Andoh,K et al.: "Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells"Biochim.Biophys.Acta. 1446. 213-224 (1999)

Andoh,K 等人：“神经胶质细胞中表达的外切磷酸二酯酶 I 基因 (PDNP3) 的基因组结构和启动子分析”Biochim.Biophys.Acta。