A Study on the Role of Protease Produced by Vibrio cholerae non-01 in the Pathogenic Mechanism of Sepsis.

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There are many reports on the cases of Vibrio cholerae infection which may extend to extra-intestinal organs. It can result in sepsis and multiple organ failure especially in immunocompromised hosts. It is then considered that a metalloprotease produced by Vibrio cholerae may be involved in its invasive mechanisms of vibrios into tissues or vessels. Because it is a permeability factor to activate kinin cascade and an activating factor of a matrix metalloprotease produced by leukocytes migrating in the inflammatory lesion. We were then trying to develop an animal model of sepsis due to Vibrio cholerae 019 to elucidate the invasive mechanisms of Vibrio cholerae. The results obtained through the study on the role of metalloprotease in pathogenic mechanisms of Vibrio cholerae and histopathological findings in sepsis model are as follows ;(1)Improvement of purification method of choleraprotease.(2)Establishment of assay system of choleraprotease using immunoblotting with ECL kit.(3)Analysis of nicking site of cholera toxin by choleraprotease.(4)Development of mouse sepsis model using Vibrio cholerae 019.(5)Involvement of choleraprotease in the pathogenic mechanism of sepsis.(6)Involvement of iNOS in the pathogenic mechanism of sepsis.