Allosteric control of collagen fibril degradation by matrix metalloprotease-1

基质金属蛋白酶-1 对胶原原纤维降解的变构控制

• 批准号: 10402052
• 负责人: Susanta Kumar Sarkar
• 金额: $ 28.46万
• 依托单位: COLORADO SCHOOL OF MINES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10402052
• 关键词: Active Sites; Affect; Allosteric Site; Animals; Atherosclerosis; Binding; Biochemical; Biological Assay; Buffers; Catalytic Domain; Cells; Collagen; Collagen Fibril; Collagen Type I; Communication; Complement; Crystallization; Diffuse; Disease; Distant; Drug usage; Dyes; Extracellular Matrix; Family; Fibrillar Collagen; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Gelatinase A; Goals; Health; Hemopexin; Home; Human; Image; Immobilization; Individual; Inflammation; Kinetics; Label; Lead; Ligand Binding; Ligands; Location; MMP9 gene; Maps; Measures; Metalloproteases; Methods; Molecular Conformation; Morphology; Motion; Mutation; Neoplasm Metastasis; Organ; Pathologic; Pathologic Processes; Physiological; Play; Pneumonia; Proteins; Proteolysis; Public Health; Relaxation; Resistance; Resolution; Role; Scanning Electron Microscopy; Side; Site; Specificity; Surface; Temperature; Testing; Tetracyclines; Time; Tissues; Variant; Water; Weight; base; experimental study; fluorescence microscope; gel electrophoresis; improved; in vitro Assay; innovation; member; molecular dynamics; monomer; mutant; public health relevance; reconstitution; scaffold; screening; simulation; single molecule; skin disorder; small molecule; therapeutic target; wound

Project Summary Collagen, the most abundant human protein, provides a scaffold for cells to maintain tissue and organ integrity. Fibrillar collagen is highly resistant to proteolysis and is degraded by specific matrix metalloproteases (MMPs). The degradation of fibrillar collagen is an essential part of tissue remodeling and is involved in many normal and pathological processes. While the degradation of triple-helical collagen monomers is well-studied, degradation of native collagen fibrils remains poorly understood. Fibrils are insoluble in physiological buffers, heterogeneous, and extended substrates, making them challenging to study using standard biochemical assays. We have overcome these limitations by developing a single-molecule method to track and analyze the motion of labeled MMPs on fibrils using a home-built total internal reflection fluorescence microscope (TIRFM). We propose to study the roles of MMP1 and MMP9 in degrading type-1 collagen fibrils. MMP1 can degrade triple-helical collagen, whereas MMP9 cannot degrade triple-helical collagen significantly. However, MMP9 is upregulated in numerous skin diseases, cancer metastasis, wound, inflammation, pneumonia, and atherosclerosis. We propose to study the mechanism of fibril degradation by two important MMPs by a combination of single-molecule tracking, innovative analysis, simulations, ensemble assays, and animal studies.

项目摘要 胶原蛋白是人类最丰富的蛋白质，它为细胞提供了维持组织和器官完整性的支架。 纤维状胶原高度抵抗蛋白质降解，并被特定的基质金属蛋白酶(MMPs)降解。 纤维性胶原的降解是组织重塑的重要组成部分，参与了许多正常和 病理过程。虽然三螺旋胶原单体的降解已经被很好地研究了，但降解 天然胶原纤维的组成仍然知之甚少。纤维不溶于生理缓冲液，是异质的， 以及延伸的底物，这使得使用标准生化分析进行研究具有挑战性。我们有 通过开发一种单分子方法来跟踪和分析标记的运动来克服这些限制 使用自制的全内反射荧光显微镜(TIRFM)在纤维上检测MMPs。我们建议 研究MMP1和MMP9在降解型胶原纤维中的作用。MMP1可降解三螺旋结构 而MMP9不能显著降解三螺旋胶原。然而，MMP9在 许多皮肤病、癌症转移、伤口、炎症、肺炎和动脉粥样硬化。我们建议 用单分子组合研究两种重要基质金属蛋白酶降解纤维的机理 跟踪、创新分析、模拟、整体分析和动物研究。