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The establishment of the Detection Method of the New Types of Influenza Virus

新型流感病毒检测方法的建立

基本信息

批准号：
10670376
负责人：
NAKAGAWA Naoko
金额：
$ 2.3万
依托单位：
Osaka Prefectural Institute of Public Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670376/
关键词：
influenza virus diagnosis epidemiology

项目摘要

1) The establishment of the rapid detection system of 2 types of influenza B virus strains. In the 1996/97 influenza season, 2 types of influenza B virus, B/Yamagata group strains and B/Victoria group stains, were isolated from the specimens of the patients. Since the standard serum was raised against a B/Yamagata group stain, B/Victoria group stains were not detected with the confirmatory diagnosis by the hemaggulutination inhibition (HI) test. We applied newly obtained monoclonal antibodies (Mabs) to a rapid diagnosis method, a PAP staining method. When anti-NP or anti-M Mabs were applied, both B/Victoria group and B/Yamagata group strains were detected equally well, and when specific anti-HA Mabs were applied, strains were clearly distinguishable.2) The establishment of the rapid detection system of hemagglutinin (HA) 5 subtype strains of influenza A virus. We immunized mice with A/Turkey/Ontario/7732/66 (H5N9) and obtained Mabs which recognize HA5 subtype strains specifically and broadly. By applying the Mabs to PAP staining method, we established the rapid diagnosis system of HA5 subtype strains.3) The establishment of the rapid detection system of HA1 subtype strains of influenza A virus. In 1999/2000 influenza season, there was an epidemic of AH1 strains in Osaka Prefecture after 4 years' absence. We are now establishing Mabs which specifically detect possible to compare the antigenicities of the strains at the next epidemics with that of the next epidemics.

1)2种B型流感病毒株快速检测体系的建立。在1996/97年流感季节，从患者标本中分离到2种B型流感病毒，即B/Yamagata群株和B/维多利亚群株。由于标准血清是针对B/Yamagata组染色剂培养的，因此通过血凝素抑制（HI）试验进行确证性诊断时未检出B/维多利亚组染色剂。我们应用新获得的单克隆抗体（Mabs）的快速诊断方法，PAP染色法。当应用抗NP或抗M单抗时，B/维多利亚群和B/Yamagata群毒株的检出率相同，而当应用特异性抗HA单抗时，毒株的检出率明显提高。2）建立了甲型流感病毒血凝素（HA）5亚型毒株的快速检测体系。我们用A/Turkey/安大略/7732/66（H5 N9）免疫小鼠，获得特异性和广泛识别HA 5亚型毒株的单克隆抗体。将单克隆抗体应用于PAP染色方法，建立了HA 5亚型毒株的快速诊断体系。3）甲型流感病毒HA 1亚型毒株快速检测体系的建立。在1999/2000年流感季节，大坂府在4年后出现了AH 1毒株的流行。我们现在正在建立特异性检测的单克隆抗体，以比较下一次流行病的菌株抗原性。