Regulation of cell surface FcεRI expression by IgE. Implication in allergy

我们研究了IL-4和IgE调节表面表达和FcεRI在碘化药物中的功能，作为一个含有大量细胞的功能。Eosinophils were purified from venous blood from consenting volunteers with no history of atopic diseases。细胞具有IL-5，有或没有IL-4和/或IgE在37℃。经过7天的培养后，用流式细胞学分析了表面FcεRI表达对碘化的表面Fc εRI mAb，CRA-1。其他外围血碘氮素没有快速表面FcεRI，但有FcεRI表达（最多占细胞FcεRI水平的1%）被观察到碘氮素，并被所有捐赠者的IL-4和IgE。IL-4和IgB的组合是表面FcεRI表达的最大定义的必要条件。在IL-4和IgE的存在下，碘氮素在2天内被培养为表面FcεRI低但有统计意义的水平，在文化7天后到达高原。如何通过CRA-1诱导碘代酚衍生的神经毒素（EDN）或leukotriene （LT） C4，或Cai-D12 + I-D1在碘代酚培养7天与IL-4和IgE。这些结果建议表面FcεRI表达在eosinophils中被调节为类似于mastcell中的机制。

We investigated whether IL-4 and IgE regulate surface expression and function of FcεRI in eosinophils, as with mast cells. Eosinophils were purified from venous blood from consenting volunteers with no history of atopic diseases. Cells were cultured with IL-5, and with or without IL-4 and/or IgE at 37℃. After culture for 7 days, the surface FcεRI expression on eosinophils was analyzed by flow cytometry using anti-FcεRI mAb, CRA-1. Although peripheral blood eosinophils did not express surface FcεRI, apparent FcεRI expression (up to 1% of mast cell FcεRI levels) was observed in eosinophils cultured with both IL-4 and IgE from all donors. The combination of IL-4 and IgB was necessary for the maximal induction of surface FcεRI expression. In the presence of IL-4 and IgE, eosinophils cultured for 2 days demonstrated low but statistically significant levels of surface FcεRI, which reached to plateau after 7 days of culture. However, cross-linking of surface FcεRI by CRA-1 failed to induce the release of eosiaophil-derived neurotoxin (EDN) or leukotriene (LT) C4, or CaィイD12+ィエD1 influx in eosinophils cultured for 7 days with IL-4 and IgE. These results suggest that surface FcεRI expression in eosinophils is regulated by a mechanism similar to that in mast cells.