Induction and the epitope analysis of human T cells specitic for human papilloma virus

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In order to investigate immune surveilence system for malignant tumors, it is important to know cellular immunity specific for tumor cells. Human papillomaviruses (HPVs) an useful tool to study cellular immune response agaist viruses and cancers induced by the viruses during the chronic infection, bscause papillomaviruses is almost established to be the cause of human cervical cancers among about 70% of the patients.We tested the cytolytic T cell (CTL) response against the truncated peptides of HPV16 E6 and E7 oncogenes from peripheral blood cells of the infected subjects detected by RT-PCR analysis of cervical cells. We could detect the CTL rsponse in 5 subjects among 150 subjects in HPV16- infected subjects. We also try to detect the minimum T cell epitopes of these CTL, and determine the HLA restriction and cell surface marker of these cells. We also induced the helper T cells specific for HPV16 E6 and E7 peptides using IL-2 response as the marker, and also detected several helper T cells specific for these petides. We try to induce the specific helper and killer clones in these cells, to know the mechanisms of T cell recognition agaist the oncogene products. We would like to study the minimum epitopes of these cells and HLA restriction to investigate the pathogenesis of cervical cancer, and the possibility of peptide vaccine for cervical neoplasms.