THE STUDY OF DNA VACCINE FOR HEPATITIS C

以质粒DNA为基础的免疫(DNA疫苗)已被证明是一种有效的动物免疫方式。应用该技术对丙型肝炎病毒DNA疫苗进行了评价。将丙型肝炎病毒核心互补DNA序列插入到巨细胞病毒早期启动子转录调控的真核表达载体中。这种免疫引起的免疫反应可能会保护实际的病毒感染。免疫Balb/c小鼠表皮。肌肉注射1/100的DNA，可在体内诱导与肌肉注射同等水平的体液免疫和细胞免疫。与肌肉注射相比，在较少的免疫频率下获得了充分的免疫应答。这是一种值得注意的新免疫方法。将该质粒DNA免疫于表达人人类白细胞抗原A2.1的转基因小鼠。在该小鼠模型中，当痘苗病毒-丙型肝炎嵌合病毒受到攻击时，DNA免疫显著抑制病毒的感染性。这是一项可能与应用于人类有关的研究。基因免疫可以作为潜在的免疫原快速构建，从而有助于丙型肝炎疫苗的发展。

Plasmid DNA-based immunization (DNA vaccine) has been shown to be an effective means of vaccination in animal mode. DNA vaccine of the hepatitis C virus (HCV) was evaluated using this technique. HCV-core complementary DNA sequences were inserted into the eukaryotic expression vector under the transcriptional control of the cytomegalovirus early promoter. The immune respose caused by this immunization may protect actual viral infection. It was immunized in the Balb/c mouse epidermis. By DNA of 1/100 quantity of intramuscular injection, both humoral and cellular immunities were induced in vivo with an equal level to intramuscular injection. Also the sufficient immune response was obtained at little immunization frequency compare to intramuscular injection. This is a method to be noticed as a new immunization. This plasmid DNA was immunized to the transgenic mouse which manifested human HLA-A2.1. The infectivity of virus was significantly suppressed by the DNA immunization, when vaccinia-HCV chimera virus was challenged in this mouse model. It is the research which may be connected with the application to the human. Genetic immunization can aid the development of HCV vaccines by allowing for the rapid construction as potential immunogens.