THE STUDY OF DNA VACCINE FOR HEPATITIS C

丙型肝炎 DNA 疫苗的研究

• 批准号: 10670447
• 负责人: SAITO Takafumi
• 金额: $ 2.3万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670447/
• 关键词: HEPATITIS C; VACCINE; IMMUNITY; PROTECTION; LYMPHOCYTE; CTL; 中和抗体

Plasmid DNA-based immunization (DNA vaccine) has been shown to be an effective means of vaccination in animal mode. DNA vaccine of the hepatitis C virus (HCV) was evaluated using this technique. HCV-core complementary DNA sequences were inserted into the eukaryotic expression vector under the transcriptional control of the cytomegalovirus early promoter. The immune respose caused by this immunization may protect actual viral infection. It was immunized in the Balb/c mouse epidermis. By DNA of 1/100 quantity of intramuscular injection, both humoral and cellular immunities were induced in vivo with an equal level to intramuscular injection. Also the sufficient immune response was obtained at little immunization frequency compare to intramuscular injection. This is a method to be noticed as a new immunization. This plasmid DNA was immunized to the transgenic mouse which manifested human HLA-A2.1. The infectivity of virus was significantly suppressed by the DNA immunization, when vaccinia-HCV chimera virus was challenged in this mouse model. It is the research which may be connected with the application to the human. Genetic immunization can aid the development of HCV vaccines by allowing for the rapid construction as potential immunogens.

基于质粒DNA的免疫（DNA疫苗）已被证明是动物模式中的有效接种手段。应用该技术对丙型肝炎病毒（HCV）DNA疫苗进行了评价。将HCV核心互补DNA序列插入到真核表达载体中，在巨细胞病毒早期启动子的转录控制下。这种免疫所引起的免疫应答可保护实际的病毒感染。在Balb/c小鼠表皮中免疫。肌肉注射1/100量的DNA，在体内诱导体液免疫和细胞免疫，其水平与肌肉注射相当。与肌肉注射相比，在较小的免疫频率下也获得了足够的免疫应答。这是一种值得注意的新免疫方法。将该质粒DNA免疫至表现人HLA-A2.1的转基因小鼠。在该小鼠模型中，当疫苗-HCV嵌合病毒攻击时，DNA免疫显著抑制病毒的感染性。这是可能与应用于人类有关的研究。基因免疫可以通过允许作为潜在免疫原的快速构建来帮助HCV疫苗的开发。

项目成果

斎藤貴史 赤塚俊隆(他): "Helios Gene GunにおけるC型肝炎ウイルスCore領域遺伝子のhaked DNA immunization"消火器と免疫. 35. 208-211 (1998)

Takashi Saito、Toshitaka Akatsuka (等人)：“Helios 基因枪中丙型肝炎病毒核心区基因的 Haked DNA 免疫”《灭火器和免疫学》35. 208-211 (1998)。

斎藤貴史、赤塚俊隆(他): "Helios Gene Gun によるC型肝炎ウィルス core 領域遺伝子の naked DNA immunization"消化器と免疫. 35. 208-211 (1998)

Takashi Saito、Toshitaka Akatsuka (等人)：“使用 Helios 基因枪对丙型肝炎病毒核心区基因进行裸 DNA 免疫”胃肠病学和免疫学。 35. 208-211 (1998)