Central mechanisms involving in responses under stress : differences between spontaneously hypertensive rats and normotensive wistar kyoto rats.

在现代压力社会中，需要弄清高血压与压力之间的关系，为高血压的治疗提供医学方法，或为解决压力提供更好的生活。本研究以自发性高血压大鼠（SHR）和正常血压的Wistar京都大鼠（WKY）为研究对象，在高血压可能是由中枢神经元异常引起的假设下，采用免疫双标（IR）方法，观察了含有谷氨酸（Glu）、谷氨酸脱羧酶（GAD）、比较了SHR和WKY两种品系的酪氨酸羟化酶（TH）、苯乙醇胺N-甲基转移酶（PNMT）和胆碱乙酰转移酶（ChAT）。在实验中，孤束核（NTS）、迷走神经背侧运动核（DMX）和疑核（AMB）内FosB/ChAT阳性神经元的数量分别为1.54、1.55、1.5 ...更多信息 SHR组显著小于WKY组。SHR大鼠延髓尾侧腹外侧区（VLM）c-Fos/TH阳性神经元数目明显多于WKY大鼠。上述结果提示，SHR DMX和AMB内较少的压力敏感性胆碱能神经元导致SHR较弱的压力感受器-心迷走神经反射，而SHR尾侧VLM内较多的压力敏感性儿茶酚胺能神经元参与了SHR较强的压力感受器-加压素反射。详细信息见第11号参考文献中的论文。另一方面，肽“爱帕琳”，这是本地化的血管壁，脂肪细胞和胃粘膜，是值得关注的。发现爱帕琳肽衍生物之一爱帕琳12具有降低血压的作用。静脉注射apelin 12对SHR的降压作用明显大于WKY。我们将研究Apelin 12在高血压和应激中生理功能的神经机制。少

In the modern stressful society, it is required to clarify relationship between the hypertension and the stress to afford the medical method for caring the hypertension or to solve the stress for the better life. Under working hypothesis that the hypertension may be caused by the abnormality in the central neurons controlling circulation, we have investigated to accumulate various neurophysiological findings by using spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY).Using the double-immunoreactive (IR) method, the topology and numbers of barosensitive neurons that contain glutamate (Glu), glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase (PNMT) and choline acetyltransferase (ChAT) were compared between the two strains of SHR and WKY. In the test experiment, the numbers of FosB/ChAT-IR neurons in the nucleus tractus solitarii (NTS), dorsal motor nucleus of vagus nerve (DMX) and ambiguus nucleus (AMB) were si … More gnificantly smaller in SHR than in WKY. The numbers of c-Fos/TH-IR neurons in the caudal ventrolateral medulla (VLM) were significantly larger in SHR than in WKY. These results suggest that a smaller number of barosensitive cholinergic neurons in the DMX and AMB in SHR causes the weaker baroreceptor-cardiac vagal reflex in SHR, and that a larger number of barosensitive catecholaminergic neurons in the caudal VLM in SHR are involved in the stronger baroreceptor-vasopressin reflex in SHR. Details were described in the paper in #11 reference. On the other hand, a peptide "apelin" , which is localized in the vascular walls, adipocytes and gastric mucosa, is worthy of attention. Apelin 12, one of the apelin peptide derivatives was found to lower blood pressure. The decrease of blood pressure by intravenous injection of apelin 12 was significantly larger in SHR than in WKY. We are going to studying the neural mechanism of the physiological function of apelin 12 with regard to the hypertension and stress. Less