Central mechanisms involving in responses under stress : differences between spontaneously hypertensive rats and normotensive wistar kyoto rats.

参与应激反应的中枢机制：自发性高血压大鼠和正常血压 Wistar Kyoto 大鼠之间的差异。

• 批准号: 10670573
• 负责人: TAKAYAMA Kiyoshige
• 金额: $ 0.45万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670573/
• 关键词: spontaneously hypertensive rat (SHR); normotensive Wistar Kyoto rat (WKY); circulation; hypertension; stress; apelin; アペリン; 胃潰瘍; シンコフェン; Fos蛋白; CRH分泌細胞; 免疫組織化学

In the modern stressful society, it is required to clarify relationship between the hypertension and the stress to afford the medical method for caring the hypertension or to solve the stress for the better life. Under working hypothesis that the hypertension may be caused by the abnormality in the central neurons controlling circulation, we have investigated to accumulate various neurophysiological findings by using spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY).Using the double-immunoreactive (IR) method, the topology and numbers of barosensitive neurons that contain glutamate (Glu), glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase (PNMT) and choline acetyltransferase (ChAT) were compared between the two strains of SHR and WKY. In the test experiment, the numbers of FosB/ChAT-IR neurons in the nucleus tractus solitarii (NTS), dorsal motor nucleus of vagus nerve (DMX) and ambiguus nucleus (AMB) were si … More gnificantly smaller in SHR than in WKY. The numbers of c-Fos/TH-IR neurons in the caudal ventrolateral medulla (VLM) were significantly larger in SHR than in WKY. These results suggest that a smaller number of barosensitive cholinergic neurons in the DMX and AMB in SHR causes the weaker baroreceptor-cardiac vagal reflex in SHR, and that a larger number of barosensitive catecholaminergic neurons in the caudal VLM in SHR are involved in the stronger baroreceptor-vasopressin reflex in SHR. Details were described in the paper in #11 reference. On the other hand, a peptide "apelin" , which is localized in the vascular walls, adipocytes and gastric mucosa, is worthy of attention. Apelin 12, one of the apelin peptide derivatives was found to lower blood pressure. The decrease of blood pressure by intravenous injection of apelin 12 was significantly larger in SHR than in WKY. We are going to studying the neural mechanism of the physiological function of apelin 12 with regard to the hypertension and stress. Less

在现代压力社会中，需要明确高血压与压力之间的关系，从而提供治疗高血压的医学方法，或者解决压力，改善生活。在高血压可能是由控制循环的中枢神经元异常引起的假设下，我们对自发性高血压大鼠（SHR）和正常Wistar Kyoto大鼠（WKY）进行了神经生理学研究。采用双免疫反应（IR）法比较了两株SHR和WKY中含有谷氨酸（Glu）、谷氨酸脱羧酶（GAD）、酪氨酸羟化酶（TH）、苯乙醇胺n -甲基转移酶（PNMT）和胆碱乙酰转移酶（ChAT）的压敏神经元的拓扑结构和数量。在实验中，大鼠孤束核（NTS）、迷走神经背运动核（DMX）和模棱两可核（AMB）中的FosB/ChAT-IR神经元数量明显少于对照组。SHR大鼠尾侧腹外侧髓质（VLM）中c-Fos/TH-IR神经元数量明显多于WKY大鼠。这些结果表明，SHR中DMX和AMB中少量的压力敏感胆碱能神经元导致SHR中较弱的压力感受器-心脏迷走反射，SHR中尾侧VLM中大量的压力敏感儿茶酚胺能神经元参与SHR中较强的压力感受器-血管加压素反射。详细信息在#11参考文献中的论文中描述。另一方面，一种定位于血管壁、脂肪细胞和胃粘膜的肽“apelin”值得关注。Apelin 12，一种Apelin肽衍生物被发现可以降低血压。静脉注射apelin 12后，SHR组血压下降幅度明显大于WKY组。我们将研究apelin 12在高血压和应激中的生理功能的神经机制。少

项目成果

Xiong Y.,Okada J.,Tomizawa S.,Takayama K.Miura M.: "Difference in topology and numbers of barosensitive catecholaminergic and cholinergic neurons in the medulla between SHR and WKY rats"Journal of the Autonomic Nervous System. 70. 200-208 (1998)

Xiong Y.，Okada J.，Tomizawa S.，Takayama K.Miura M.：“SHR 和 WKY 大鼠髓质中压力敏感儿茶酚胺能和胆碱能神经元的拓扑和数量差异”自主神经系统杂志。

Xiong, Y., Okada, J., Tomizawa, S., Takayama, K., Miura, M.: "Difference in topology and numbers of barosensitive catecholaminergic and cholinergic neurons in the medulla between SHR and WKY rats."Journal of the Autonomic Nervous System. 70. 200-208 (1998

Xiong, Y.、Okada, J.、Tomizawa, S.、Takayama, K.、Miura, M.：“SHR 和 WKY 大鼠髓质中压力敏感儿茶酚胺能和胆碱能神经元的拓扑结构和数量差异。”

Xiong,Y.,Okada,J.,Tomizawa,S.,Takayama,K.,Miura,M.: "Difference in topology and numbers of barosensitive catecholaminergic and cholinergic neurons in the medulla between SHR and WKY rats"Journal of the Autonomic Nervous System. 70. 200-208 (1998)

Xiong,Y.,Okada,J.,Tomizawa,S.,Takayama,K.,Miura,M.：“SHR 和 WKY 大鼠髓质中压力敏感儿茶酚胺能和胆碱能神经元的拓扑结构和数量差异”自主神经杂志

Xiong. Y. ,Okada. J. ,Tomizawa. S. ,Takayama. K. ,Miura,M.: "Difference in topology and numbers of barosensitive catecholaminergic and cholinergic neurons in the medulla between SHR and WKY rats." Journal of the Autonomic Nervous System. 70. 200-208 (1998

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