Molecular epidemiological study of Haemophilus influenza serotype b strains of tained from children with meningitis.

我们报告了一项从脑膜炎儿童脑脊液中提取的30株b型流感嗜血杆菌（Hib）的流行病学研究。对Hib菌株进行生物分型，检测其β-内酰胺酶的产生，并通过长pcr -核糖分型、随机扩增多态性DNA （RAPD）分析和基因组DNA限制性片段长度多态性（RFLP）分析进行基因分型（脉冲场凝胶电泳（PFGE））。菌株的表型特征研究22(73%)和生物型。基因型的研究使用长PCR-ribotyping HaeIII限制消化没有多态性其中30 b型流感嗜血杆菌菌株,但与两组引物RAPD分析演示了两个独特的亚型:一个典型的菌株生物型组II和第二株生物型组织的特征和IV。每个RAPD组被PFGE-RFLP子类型分成几个基因型组与SmaI消化。临床分离Hib菌株的基因分型可能有助于阐明社区感染的传播途径、地方性和疫苗失败的原因。

We report an epidemiological study of 30 Haemophilus influenzae serotype b (Hib) strains derived from the cerebrospinal fluid of children with meningitis. The Hib strains were biotyped, tested for β-lactamase production, and genotyped by long PCR-ribotyping, random amplified polymorphic DNA (RAPD) analysis, and genomic DNA restriction fragment length polymorphism (RFLP) analysis by pulsed-field gel electrophoresis (PFGE). The phenotypic study characterized 22 of the strains (73%) as biotype I. A genotypic study using long PCR-ribotyping with HaeIII restriction digestion showed no polymorphisms among these 30 Hib strains, but RAPD analysis with two sets of primers demonstrated two distinctive subtypes : one typical of the strains of biotype group II and the second characteristic of the strains of biotype groups I and IV. Each RAPD group was subtyped into several genotypic groups by PFGE-RFLP with SmaI digestion. The genotyping of clinically isolated Hib strains may help to elucidate transmission routes in community infections, endemicity, and the reasons for vaccine failure.