Analysis of monoclonal T cells in benign dermatoses related to mycosis fungoides.

与蕈样肉芽肿相关的良性皮肤病中的单克隆 T 细胞分析。

• 批准号: 10670790
• 负责人: HAGARI Yoshitaka
• 金额: $ 1.54万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670790/
• 关键词: Pityriasis lichenoides; PCR; TCR; Monoclonality; In-situ hybridization; Immunohistochemistry; Surfase marker; T cell; T細胞受容体; 免疫グロブリン遺伝子; 菌状息肉症; 急性痘瘡状苔蘚状粃糠疹; 悪性リンパ腫

Pityriasis lichenoides has been regarded as a benign inflammatory disorder belonging to the parapsoriasis group. However, based on its association with cutaneous T cell lymphoma (CTCL) or the presence of monoclonal populations in infiltrates, it has been hypothesized that pityriasis lichenoides is part of the CTCL disease sectrum. For validation of this hypothesis, the monoclonal populations in pityriasis lichenoides should express the same phenotype as CTCL CID4^+ Tlymphocyte.In this study, we analysed infiltrates in 7 cases of pityriasis lichenoides (6 cases of pityriasis lichenoides et varioliformis acuta and one case of pityriasis lichenoides chronica) using praffin-embedded specimens.1. By amplifying the rearranged TCR-γ genes, monoclonal populations were detected in 6 of 7 cases of pityriasis lichenoides.2. These amplified bands were recovered and sequenced. These data showed that the PCR-based method adequately amplified the rearranged TCR-γ genes and specified the gene segments used in the rearrangement processes.3. We prepared pairs of mirror sections that gave the same histological picture as a mirror image. In each pair, in-situ hybridization (ISH) using the amplified band as a probe on one section and immunohistochemistry using UCHL1, anti-CD8, or anti-CD4 antibodies on the other section were performed. By comparing these sections, the phenotypes of the ISH-positive cells were determined as UCHL1^+, CD8^-, CD4^+ cells.These results demonstrated that pityriasis lichenoides qualified as a member of parapsoriasis. Furthermore, we suggest that the interrelationship between monoclonal CD4^+ cells and reactive CD8^+ cells affects the biological behavior of the disease in the CTCL disease spectrum.

地衣样糠疹一直被认为是一种良性炎性疾病，属于副银屑病组。然而，基于其与皮肤T细胞淋巴瘤（CTCL）的关联或浸润中单克隆群体的存在，人们假设地衣样糠疹是CTCL疾病谱的一部分。为了验证这一假设，地衣糠疹的单克隆群体应该表达与CTCL CID4^+ t淋巴细胞相同的表型。本研究采用石蜡包埋标本对7例地衣样糠疹（6例急性变型地衣样糠疹和1例慢性地衣样糠疹）的浸润情况进行了分析。通过扩增重排的TCR-γ基因，在7例地衣样糠疹患者中检测到单克隆群体。这些扩增的条带被恢复并测序。这些数据表明，基于pcr的方法充分扩增了重排的TCR-γ基因，并指定了重排过程中使用的基因片段。我们准备了成对的镜像切片，提供了与镜像相同的组织学图像。在每一对中，用扩增条带作为探针在一个切片上进行原位杂交（ISH），在另一个切片上使用UCHL1、抗cd8或抗cd4抗体进行免疫组化。通过比较这些切片，确定了ish阳性细胞的表型为UCHL1^+， CD8^-， CD4^+细胞。这些结果表明，地衣状糠疹是银屑病的一个成员。此外，我们认为单克隆CD4^+细胞和反应性CD8^+细胞之间的相互关系影响了CTCL疾病谱系中疾病的生物学行为。