Fundamental Study on Assessment of Neurotransplantation and Drug Treatment in Alzheimers Disease Using Nuclear Medicine Imaging

(1)将β-淀粉样蛋白注入大鼠脑室14天，用八臂放射状迷宫评价大鼠的记忆能力。在相同的动物中，测量囊泡乙酰胆碱转运体和M胆碱受体密度。在β-淀粉样蛋白处理的大鼠中，八臂径向迷宫任务的执行受到损害。在新皮质，β-淀粉样蛋白处理的大鼠的囊泡乙酰胆碱转运体密度低于赋形剂处理的大鼠；M受体密度在两组之间没有差异。这些结果提示，囊泡型乙酰胆碱转运体密度的降低与β-淀粉样蛋白引起的记忆障碍有关。(2)结状神经节移植可改善大鼠学习记忆障碍，[~3H]-维沙米考是突触前胆碱能神经元的标记剂之一。用高灵敏显像板系统进行定量放射自显影，评价神经移植对[~(3 H)]-维沙米考结合的影响。损毁大细胞基底核后1、2、4周，维沙米考的患侧/健侧比值明显低于假手术组(P<0.01)。移植组4周时显著高于未治疗组(p&lt；0.05)。-Vesamicol结合是判断大细胞基底核病变和结节移植效果的最敏感指标之一。

(1) In this study, β-amyloid protein was infused into the cerebral ventricle of rats for 14 days ; the eight-arm radial maze was used to evaluate memorial ability. In the same animals, vesicular acetylcholine transporter and muscarinic acetylcholine receptor density was measured. The performance of the eight-arm radial maze task was impaired in β-amyloid protein treated rats. In neocortex, the vesicular acetylcholine transporter density was lower in β-amyloid protein-treated rats than vehicle treated rats ; there was no difference in muscarinic acetylcholine receptor density between the two groups. These results suggest that the reduction in vesicular acetylcholine transporter density is related to memory impairment induced by β-amyloid protein.(2) It is considered that the nodosal ganglion grafting improves the learning and memory disorder in the nucleus basalis magnocellularis lesioned rat, and that [^3H]-vesamicol is one of the mapping agents for presynaptic cholinergic neurons. Quantitative autoradiography was performed using high sensitive imaging plate system to evaluate the effect of neurotransplantation on [^3H]-vesamicol binding. The affected-side to unaffected-side ratio of [^3H]-vesamicol in the nucleus basalis magnocellularis lesioned group was significantly lower than that in the sham-operated group at I , 2 and 4 weeks (p< 0.01). In the transplanted group, the ratio was significantly higher than that in the untreated group at 4 weeks (p<0.05). [^3H]-vesamicol binding is one of the most sensitive indicators of nucleus basalis magnocellularis lesions and the effect of the nodosal ganglion grafting.