A Study of a Quantification of Organs Perfusion with Animal Model Using Nuclear Medicine Technique : Factor of Absorption and Scatter on Gamma-Ray

利用核医学技术对动物模型器官灌注进行定量研究：伽马射线吸收和散射因素

• 批准号: 10670836
• 负责人: ONOGUCHI Masahisa
• 金额: $ 0.77万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670836/
• 关键词: ィイD199mィエD1Tc-tetrofosmin; Myocardial uptake; Planar images; Scintillation counter; Rat; ガンマカメラ; テトロフオスミン; 心筋血流; 定量; 吸収; 散乱線

It's reported that the duodenum and the small intestine of the activity excreted from the liver and gallbladder can lead to interference with the myocardial uptake. To study how precisely images reflect tracer uptake in a myocardium, as fundamental experiment to examine the influence that accumulation of the liver and alimentary tract (except esophagus) of the activity excreted from the liver gave to the myocardium image in the technetium-99m myocardium perfusion tracer, we administered ィイD199mィエD1Tc-tetrofosmin (following, Tetrofosmin) to a normal rat and compared with measured value obtained by a gamma camera and an actual survey value after the organs extraction. Methods : Thirty-three rats were measured for 5, 10, 15, 30, 45, 60, 90 and 120min after the administration of Tetrofosmin. The accumulated rates for the myocardium, the lung, the liver, the duodenum, the small intestine, the rectum and the blood with a well-type scintillation counter (WC) were assessed as a percentage of t … More he injected dose per gram of tissue (%ID/g). The accumulated rates for each organ with the planar imaging (PI) were assessed as a percentage of the injected dose per pixel (%ID/pixel) establishing regions of interest (ROIs) in each organ. The accumulated rates for each organ, the PI-to-WC ratio and the heart-to-organ ratios calculated by PI and WC were compared. Results : The accumulated rate for the heart with WC increased until 30 min (1.671%±0.306%) after the injection, and subsequently decreased. On the other hand, peak of the accumulated rate with PI was shown at 15 min (1.814%±0.518%) after the injection. Particularly a difference of both was higher in 10 min (PI/WC ratio : about 1.6 times) of the early phase. The accumulated rate for the lung with WC was maximum at 15 min (0.870%±0.312%) after the injection. The accumulated rate with PI, however, showed the maximum value at 10 min (1.139%±0.376%), and a variation of the accumulated rate was hardly recognized 10 min later. The accumulated rate for the liver with WC was hardly shown a marked variance from 5min (0.768%±0.193%) to 30min (0.692%±0.273%), and a tendency of decrease was showed slowly. On the other hand, the accumulated rate with PI was more highly than that with WC in the whole, and high values were showed in 10 min (PI/WC ratio : about 3.3 times) and 15 min (PI/WC ratio : about 2.3 times) in particular. The accumulated rate for the duodenum and the upper small intestine was maximum in 10 min (1.228%±0.602%, 2.245%±1.936%) after the injection, and approximately 90% of ィイD199mィエD1Tc-tetrofosmin were the duodenum excrements. Conclusion : The accumulated rates for each organ with PI were showed higher than that with WC. The difference of both, particularly, was stronger in early phase after the injection. The accumulated rate for the heart with PI was shown significant higher than that with WC at 10 min. The interference of Compton scatter from the liver, duodenum and small intestine was suggested as one of these causes. Less

据报道，十二指肠和小肠的活动从肝脏和胆囊排出，可导致干扰心肌摄取。研究图像如何准确地反映心肌中示踪剂的摄取，作为检查肝脏和消化道积聚对心肌的影响的基础实验（食管除外）的活动，从肝脏排泄给心肌显像的锝-99m心肌灌注示踪剂，我们给予199 m锝D1 Tc-替曲膦（以下，Tetrofosmin）对正常大鼠进行测量，并与通过γ照相机获得的测量值和器官提取后的实际测量值进行比较。方法：33只大鼠分别于给药后5、10、15、30、45、60、90和120 min测定脑组织中的钙离子浓度。用井式闪烁计数器（WC）测定心肌、肺、肝、十二指肠、小肠、直肠和血液的累积率，以占总累积率的百分比表示。 ...更多信息 每克组织的注射剂量（%ID/g）。用平面成像（PI）评估每个器官的累积速率，作为每个器官中建立感兴趣区域（ROI）的每个像素注射剂量的百分比（%ID/像素）。比较了每个器官的累积速率、PI与WC的比值以及通过PI和WC计算的心脏与器官的比值。结果：WC组心脏的累积心率在注射后30 min开始增加（1.671%±0.306%），随后逐渐下降;与PI的累积速率在注射后15 min出现峰值（1.814%±0.518%）。特别是在早期阶段的10分钟（PI/WC比：约1.6倍）两者的差异更高。WC在肺内的蓄积率在注药后15 min达到最大值（0.870%±0.312%）。而与PI的蓄积率在10 min时达到最大值（1.139%±0.376%），10 min后蓄积率几乎没有变化。肝内WC蓄积率在5 min（0.768%±0.193%）~ 30 min（0.692%±0.273%）变化不大，呈缓慢下降趋势。另一方面，PI的累积速率总体上高于WC，特别是在10 min（PI/WC比：约3.3倍）和15 min（PI/WC比：约2.3倍）显示出高值。注射后10 min，在十二指肠和小肠上段的蓄积率最高（1.228%± 0.602%，2.245%±1.936%），约90%的D199 m锝-替曲膦在十二指肠排泄。结论：PI组各器官的累积率均高于WC组。尤其是在注射后的早期阶段，两者的差异更大。10 min时，PI组心脏的累积速率明显高于WC组，肝、十二指肠和小肠的康普顿散射干扰是其原因之一。少

项目成果

小野口昌久: "99mTC-tetrofosmin心筋画像はどの程度真の取り込みを反映しているか?―ラットを用いたガンマカメラによる測定と実測値の対比―"北陸循環核医学研究会記録集. 31. 5-6 (1999)

Masahisa Onoguchi：“99mTC-替曲膦心肌图像在多大程度上反映了真实的摄取？ - 伽马相机测量值与大鼠实际测量值的比较 -” 北陆循环核医学研究组记录 31. 5-6 (1999)。

Onoguchi, Masahisa: "Biokinetics of Technetium-99m-Tetrofosmin Myocardial Perfusion Imaging Agent in Rats : Comparison between the Values from Planar Images and Excised Organs"Memoirs Health Sci Med Kanazawa Univ. 23. 47-55 (1999)

Onoguchi，Masahisa：“大鼠体内 Technetium-99m-Tetrofosmin 心肌灌注成像剂的生物动力学：平面图像和切除器官的值之间的比较”回忆录健康科学医学金泽大学。

小野口昌久: "ラットにおける心筋四流製剤99mTc-tetrofosminの体内分布―プラナー像と摘出臓器による値の対比―"金沢大学医学部保健学科紀要. 23・2. 47-55 (1999)

小野口正久：“心肌四流制剂99mTc-替曲膦在大鼠体内的分布-平面图像与提取器官的值的比较-”金泽大学医学院健康科学系通报23, 2. 47。 -55 (1999)

小野口昌久: "^<99m>Tc-Tetrofosmin心筋画像はどの程度真の取り込みを反映しているか?-ラットを用いたガンマカメラによる測定と実測値の対比-"北陸循環器核医学研究会記録集. 31. 5-6 (1999)

小野口正久：“^<99m>Tc-替曲膦的心肌图像在多大程度上反映了真实的摄取？-使用大鼠的伽马相机测量与实际测量的比较-”北陆心血管核医学研究组记录31. 5。 -6 (1999)

Onoguchi, Masahisa: "How precisely Myocardial Perfusion images reflect racer uptake? : Comparison between the Values from Planar Images and Excised Organs in Rats"Hokuriku Nuclear Medicine Circulation Conference. 31. 5-6 (1999)

Onoguchi，Masahisa：“心肌灌注图像如何精确地反映赛车摄取？：平面图像和大鼠切除器官的值之间的比较”北陆核医学循环会议。