MONITORING OF T-CELL RESEPTOR COMPLEMENTARITY-DETERMINIG REGION 3(CDR3)DIVERSITY AFTER HUMAN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION

我们研究了异基因造血干细胞移植后t细胞受体库的重建。我们发现在移植后早期观察到t细胞受体-β链可变区（TCRBV）和TCR-α链可变区（TCRAV）的扭曲库，并且在成人中扭曲库的正常化需要数年时间。在儿童患者中，TCRBV和AV使用的偏斜幅度较小，偏斜曲目的正常化在儿童中比在成人中更快。T淋巴细胞的广泛克隆扩增引起移植后TCR库的倾斜，并且克隆扩增的T淋巴细胞在CD8+CD28-部分中富集。TCR-β和-δ链的CDR3大小谱型分析显示，受体的免疫能力与TCRαβ+的库多样性重构有关，而与notcr γδ+的库多样性重构有关。这些结果提示外周血t细胞扩增可能是造血干细胞移植后t细胞再生的主要机制。

We investigated the reconstitution of T-cell receptor repertoire after human allogeneic hematopoietic stem cell transplantation. We found that skewed repertoires of the T-cell receptor-β chain variable region(TCRBV)and the TCR-α chain variable region(TCRAV)were observed at an early period after transplantation and that normalization of skewed repertoires required several years in adults. The magnitude of skewing in the usage of TCRBV and AV was small in pediatric patients and normalization of skewed repertoires was faster in children than in adults. Extensive clonal expansion of T lymphocytes caused post-transplant skewing of TCR repertoires and clonally expanded T lymphocytes were enriched in the CD8+CD28- fraction. CDR3 size spectratyping analysis for the TCR-β and -δ chains revealed that immunocompetence in recipients was associated with the reconstitution of repertoire diversity of TCRαβ+, but notTCRγδ+, T lymphocytes. These results suggest that peripheral T-cell expansion may be the principal mechanism of T-cell regeneration after hematopoietic stem cell transplantation.