Role for γδT lymphocytes in autoimmune bone marrow failure syndrome

γδT 淋巴细胞在自身免疫性骨髓衰竭综合征中的作用

• 批准号: 22591024
• 负责人: HIROKAWA Makoto
• 金额: $ 2.83万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591024/
• 关键词: 赤芽球癆; 後天性; 慢性; γδT細胞; CDR3; 骨髄不全症候群

We examined the γδ T cell receptor repertoire in 19 pure red cell aplasia (PRCA) patients and found that there was a difference in γδ T-cell repertoires between PRCA patients and healthy donors. We observed an increase in Vδ1 γδ T cells and a decrease in Vδ2 T cells in PRCA patients. An increase of Vδ1 γδ T cells was not found in aplastic anemia patients. CDR3.1 size distribution patterns were skewed in 9 out of 13 PRCA patients examined, although the skewing was also observed in 7 out of 10 healthy individuals. No significant changes were present in CDR3.1 size distribution between PRCA patients and healthy donors. Moreover, no apparent consensus amino acid motifs were identified in PRCA patients. Expansion of Vδ1 T cells and depletion of Vδ2 T cells are unique features for chronic acquired PRCA but expansion of Vδ1 T cells does not seem to be the consequence of CDR3-dependent selection. We conclude that clonal expansion of Vδ1 T cells is not a general feature for chronic acquired PRCA.

我们检测了19例纯红细胞再生障碍性贫血（PRCA）患者的γδ T细胞受体谱，发现PRCA患者和健康供体之间的γδ T细胞受体谱存在差异。我们观察到PRCA患者中Vδ1 γδ T细胞增加，Vδ2 T细胞减少。再生障碍性贫血患者外周血中Vδ1 γδ T细胞无明显增加。CDR3.1大小分布模式在检查的13名PRCA患者中的9名中是偏斜的，尽管在10名健康个体中的7名中也观察到偏斜。PRCA患者和健康供体之间的CDR3.1大小分布没有显著变化。此外，在PRCA患者中未发现明显的共有氨基酸基序。Vδ1 T细胞的扩增和Vδ2 T细胞的耗竭是慢性获得性PRCA的独特特征，但Vδ1 T细胞的扩增似乎不是CDR 3依赖性选择的结果。我们得出结论，Vδ1 T细胞的克隆性扩增不是慢性获得性PRCA的一般特征。

项目成果

Effect of oral itraconazole on the pharmacokinetics of tacrolimus in a HSCT patient with CYP3A5*3/*3

口服伊曲康唑对 CYP3A5*3/*3 HSCT 患者他克莫司药代动力学的影响

• 发表时间: 2010
• 作者: Nara M;et al.
• 通讯作者: et al.

血液診療エキスパート:赤芽球癆(後天性)

血液治疗专家：成红细胞再生障碍性贫血（获得性）

• 发表时间: 2010
• 作者: 廣川誠;澤田賢一;廣川 誠;廣川 誠
• 通讯作者: 廣川 誠

CpG-ODN 2006 and human parvovirus B19 genome consensus sequences selectively inhibit growth and development of erythroid progenitor cells

• DOI: 10.1182/blood-2009-08-239202
• 发表时间: 2010-06-03
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Guo, Yong-Mei;Ishii, Keiko;Sawada, Kenichi
• 通讯作者: Sawada, Kenichi

CDR3-independent expansion of Vδ1 T lymphocytes in acquired chronic pure red cell aplasia

• DOI: 10.1016/j.imlet.2012.12.006
• 发表时间: 2013-02-01
• 期刊: IMMUNOLOGY LETTERS
• 影响因子: 4.4
• 作者: Michishita, Yoshihiro;Hirokawa, Makoto;Sawada, Kenichi
• 通讯作者: Sawada, Kenichi

Alteration of γδ T-cell repertoire in acquired chronic pure red cell aplasia

获得性慢性纯红细胞再生障碍性贫血中 γδ T 细胞库的改变

• 发表时间: 2010
• 作者: Hirokawa M;Michishita Y;Fujishima N;Fujishima M;Guo YM;Kameoka Y;Saitoh H;Tagawa H;Takahashi N;Sawada K
• 通讯作者: Sawada K